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https://hdl.handle.net/2440/8343
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dc.contributor.author | Robertson, S. | - |
dc.contributor.author | OConnell, A. | - |
dc.contributor.author | Hudson Keenihan, S. | - |
dc.contributor.author | Seamark, R. | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Journal of Reproductive Immunology, 2000; 46(2):131-154 | - |
dc.identifier.issn | 0165-0378 | - |
dc.identifier.issn | 1872-7603 | - |
dc.identifier.uri | http://hdl.handle.net/2440/8343 | - |
dc.description.abstract | Factors in seminal plasma elicit a surge of GM-CSF expression in uterine epithelial cells after mating in mice. This study investigates the nature of the endometrial cell populations targeted by epithelial GM-CSF. In quantitative RT-PCR studies, expression of the alpha-subunit of the GM-CSF receptor (GM-CSF-R) parallelled GM-CSF expression, being maximal during the 48 h period after mating and declining thereafter. Expression of mRNA encoding beta-common chain (AIC2B) also increased after mating and remained high until the time of embryo implantation on day 4 of pregnancy. Cells expressing GM-CSF receptors were identified in sections of uterus on the day after mating using 125I-GM-CSF, and were located predominantly in the endometrial stroma subjacent to the luminal epithelium, co-localising with abundant populations of myeloid leukocytes. Cells expressing GM-CSF receptor were identified as macrophages, granulocytes and putative dendritic cells by flow cytometric analysis using lineage and receptor subunit specific antibodies. Recombinant GM-CSF injected into the uterine lumen of ovariectomised mice was found to elicit a dose-dependant accumulation of macrophages and granulocytes in the endometrium, in a pattern of distribution comparable to that seen in uteri after natural mating. Together, these data indicate a role for epithelial cell-derived GM-CSF in mediating the recruitment and potentially in modifying the behaviour of uterine leukocytes during the post-mating inflammatory response in mice. | - |
dc.description.statementofresponsibility | Sarah A Robertson, Aishling C O’Connell, Sarah N Hudson, Robert F Seamark | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Sci Ireland Ltd | - |
dc.source.uri | http://dx.doi.org/10.1016/s0165-0378(99)00060-1 | - |
dc.subject | Macrophage | - |
dc.subject | Dendritic cell | - |
dc.subject | Neutrophil | - |
dc.subject | Eosinophil | - |
dc.subject | GM-CSF | - |
dc.subject | GM-CSF receptor | - |
dc.subject | Uterus | - |
dc.title | Granulocyte-macrophage colony-stimulating factor (GM-CSF) targets myeloid leukocytes in the uterus during the post-mating inflammatory response in mice | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/S0165-0378(99)00060-1 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Robertson, S. [0000-0002-9967-0084] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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