Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
Full metadata record
|dc.identifier.citation||Journal of Biological Chemistry, 2012; 287(16):12952-12965||en|
|dc.description.abstract||We have provided the first evidence for specific heteromerization between the α1A-adrenoceptor (α1AAR) and CXC chemokine receptor 2 (CXCR2) in live cells. α1AAR and CXCR2 are both expressed in areas such as the stromal smooth muscle layer of the prostate. By utilizing the G protein-coupled receptor (GPCR) heteromer identification technology on the live cell-based bioluminescence resonance energy transfer (BRET) assay platform, our studies in human embryonic kidney 293 cells have identified norepinephrine-dependent β-arrestin recruitment that was in turn dependent upon co-expression of α1AAR with CXCR2. These findings have been supported by co-localization observed using confocal microscopy. This norepinephrine-dependent β-arrestin recruitment was inhibited not only by the α1AR antagonist Terazosin but also by the CXCR2-specific allosteric inverse agonist SB265610. Furthermore, Labetalol, which is marketed for hypertension as a nonselective β-adrenoceptor antagonist with α1AR antagonist properties, was identified as a heteromer-specific-biased agonist exhibiting partial agonism for inositol phosphate production but essentially full agonism for β-arrestin recruitment at the α1AAR-CXCR2 heteromer. Finally, bioluminescence resonance energy transfer studies with both receptors tagged suggest that α1AAR-CXCR2 heteromerization occurs constitutively and is not modulated by ligand. These findings support the concept of GPCR heteromer complexes exhibiting distinct pharmacology, thereby providing additional mechanisms through which GPCRs can potentially achieve their diverse biological functions. This has important implications for the use and future development of pharmaceuticals targeting these receptors.||en|
|dc.description.statementofresponsibility||Sanam Mustafa, Heng B. See, Ruth M. Seeber, Stephen P. Armstrong, Carl W. White, Sabatino Ventura, Mohammed Akli Ayoub, and Kevin D. G. Pfleger||en|
|dc.publisher||Amer Soc Biochemistry Molecular Biology Inc||en|
|dc.rights||© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.||en|
|dc.subject||7-Helix Receptor; Adrenergic Receptor; Allosteric Regulation; Arrestin; Chemokines; G Protein-coupled Receptors (GPCR); Heteromer||en|
|dc.title||Identification and profiling of novel a1A-adrenoceptor-CXC chemokine receptor 2 heteromer||en|
|pubs.library.collection||Medical Sciences publications||en|
|dc.identifier.orcid||Mustafa, S. [0000-0002-8677-5151]||en|
|Appears in Collections:||Medical Sciences publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.