Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/83556
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dc.contributor.authorAtaide, R.en
dc.contributor.authorHasang, W.en
dc.contributor.authorWilson, D.en
dc.contributor.authorBeeson, J.en
dc.contributor.authorMwapasa, V.en
dc.contributor.authorMolyneux, M.en
dc.contributor.authorMeshnick, S.en
dc.contributor.authorRogerson, S.en
dc.date.issued2010en
dc.identifier.citationPLoS One, 2010; 5(5):e10807-1-e10807-10en
dc.identifier.issn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.urihttp://hdl.handle.net/2440/83556-
dc.description.abstractBackground: Pregnant women residing in malaria endemic areas are highly susceptible to Plasmodium falciparum malaria, particularly during their first pregnancy, resulting in low birth weight babies and maternal anaemia. This susceptibility is associated with placental sequestration of parasitised red blood cells expressing pregnancy-specific variant surface antigens. Acquisition of antibodies against these variant surface antigens may protect women and their offspring. Functions of such antibodies may include prevention of placental sequestration or opsonisation of parasitised cells for phagocytic clearance. Methodology/Findings: Here we report the development and optimisation of a new high-throughput flow cytometry-based phagocytosis assay using undifferentiated Thp-1 cells to quantitate the amount of opsonizing antibody in patient sera, and apply this assay to measure the impact of HIV on the levels of antibodies to a pregnancy malaria-associated parasite line in a cohort of Malawian primigravid women. The assay showed high reproducibility, with inter-experimental correlation of r2 = 0.99. In primigravid women, concurrent malaria infection was associated with significantly increased antibodies, whereas HIV decreased the ability to acquire opsonising antibodies (Mann-Whitney ranksum: p = 0.013). This decrease was correlated with HIV-induced immunosuppression, with women with less than 350×106 CD4+ T- cells/L having less opsonising antibodies (coef: −11.95,P = 0.002). Levels of antibodies were not associated with protection from low birth weight or anaemia. Conclusions/Significance: This flow cytometry-based phagocytosis assay proved to be efficient and accurate for the measurement of Fc-receptor mediated phagocytosis-inducing antibodies in large cohorts. HIV was found to affect mainly the acquisition of antibodies to pregnancy-specific malaria in primigravidae. Further studies of the relationship between opsonising antibodies to malaria in pregnancy and HIV are indicated.en
dc.description.statementofresponsibilityRicardo Ataíde, Wina Hasang, Danny W. Wilson, James G. Beeson, Victor Mwapasa, Malcolm E. Molyneux, Steven R. Meshnick, Stephen J. Rogersonen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.isreplacedby2440/89935-
dc.relation.isreplacedbyhttp://hdl.handle.net/2440/89935-
dc.rights© 2010 Ataíde et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectCell Line; Humans; HIV; HIV Infections; Pregnancy Complications, Parasitic; Malaria, Falciparum; Anemia; Hemoglobins; Immunoglobulin G; Antibodies, Protozoan; Antigens, Protozoan; Immunoassay; Cohort Studies; Cell Differentiation; Phagocytosis; Antibody Specificity; Pregnancy; Infant, Newborn; Infant, Low Birth Weight; Femaleen
dc.titleUsing an improved phagocytosis assay to evaluate the effect of HIV on specific antibodies to pregnancy-associated malariaen
dc.typeJournal articleen
dc.identifier.rmid0020138051en
dc.identifier.doi10.1371/journal.pone.0010807en
dc.identifier.pubid14531-
pubs.library.collectionMolecular and Biomedical Science publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidWilson, D. [0000-0002-5073-1405]en
Appears in Collections:Molecular and Biomedical Science publications

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