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|Title:||Differential regulation by FSH and IGF-I of extracellular matrix IGFBP-5 in bovine granulosa cells: effect of association with the oocyte|
|Citation:||Molecular and Cellular Endocrinology, 2000; 164(1-2):53-58|
|Publisher:||Elsevier Sci Ireland Ltd|
|Abstract:||Inhibition of insulin-like growth factor (IGF)-I induced DNA synthesis in bovine oocyte-cumulus complexes (OCCs) caused by follicle-stimulating hormone (FSH) has been linked to changes in the extracellular matrix which do not occur in mural granulosa cells (MGCs). We investigated regulation by IGF-I and FSH of secreted and extracellular matrix entrapped IGF-binding proteins. OCCs and MGCs from bovine ovaries were cultured in media supplemented with IGF-I and FSH for 24 h. Culture media and extracellular matrix were analysed for IGF-binding proteins by Western ligand blot and immunoblot and found to contain principally IGFBP-3 and -5. The combined treatment of IGF-I and FSH increased the concentration of IGFBP-3 in OCC and MGC conditioned media by 4- and 6-fold, respectively. Treatment of OCCs and not MGCs with IGF-I and FSH together increased extracellular matrix IGFBP-5 by 2.5-fold. The differential regulation of extracellular matrix IGFBP-5 in OCCs compared to MGCs suggest involvement of changes in the extracellular matrix brought about by IGF-I and FSH in overall regulation of IGF-I in the ovarian follicle.|
|Keywords:||Insulin-like growth factor binding protein; Extracellular matrix; Oocyte; Cumulus oophorus; Granulosa cells; Follicle-stimulating hormone|
|Description:||Website © 2000 Ingenta. Article copyright remains with the publisher, society or author(s) as specified within the article|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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