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dc.contributor.authorIngman, W.en
dc.contributor.authorOwens, P.en
dc.contributor.authorArmstrong, D.en
dc.identifier.citationMolecular and Cellular Endocrinology, 2000; 164(1-2):53-58en
dc.descriptionWebsite © 2000 Ingenta. Article copyright remains with the publisher, society or author(s) as specified within the articleen
dc.description.abstractInhibition of insulin-like growth factor (IGF)-I induced DNA synthesis in bovine oocyte-cumulus complexes (OCCs) caused by follicle-stimulating hormone (FSH) has been linked to changes in the extracellular matrix which do not occur in mural granulosa cells (MGCs). We investigated regulation by IGF-I and FSH of secreted and extracellular matrix entrapped IGF-binding proteins. OCCs and MGCs from bovine ovaries were cultured in media supplemented with IGF-I and FSH for 24 h. Culture media and extracellular matrix were analysed for IGF-binding proteins by Western ligand blot and immunoblot and found to contain principally IGFBP-3 and -5. The combined treatment of IGF-I and FSH increased the concentration of IGFBP-3 in OCC and MGC conditioned media by 4- and 6-fold, respectively. Treatment of OCCs and not MGCs with IGF-I and FSH together increased extracellular matrix IGFBP-5 by 2.5-fold. The differential regulation of extracellular matrix IGFBP-5 in OCCs compared to MGCs suggest involvement of changes in the extracellular matrix brought about by IGF-I and FSH in overall regulation of IGF-I in the ovarian follicle.en
dc.publisherElsevier Sci Ireland Ltden
dc.subjectInsulin-like growth factor binding protein; Extracellular matrix; Oocyte; Cumulus oophorus; Granulosa cells; Follicle-stimulating hormoneen
dc.titleDifferential regulation by FSH and IGF-I of extracellular matrix IGFBP-5 in bovine granulosa cells: effect of association with the oocyteen
dc.typeJournal articleen
pubs.library.collectionObstetrics and Gynaecology publicationsen
dc.identifier.orcidIngman, W. [0000-0003-3116-2902]en
Appears in Collections:Obstetrics and Gynaecology publications

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