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Type: Journal article
Title: A human, double-blind, placebo-controlled, crossover trial of prebiotic, probiotic, and synbiotic supplementation: Effects on luminal, inflammatory, epigenetic, and epithelial biomarkers of colorectal cancer
Author: Worthley, D.
Le Leu, R.
Whitehall, V.
Conlon, M.
Christophersen, C.
Belobrajdic, D.
Mallitt, K.
Hu, Y.
Irahara, N.
Ogino, S.
Leggett, B.
Young, G.
Citation: American Journal of Clinical Nutrition, 2009; 90(3):578-586
Publisher: American Society for Nutrition
Issue Date: 2009
ISSN: 1938-3207
Statement of
Daniel L Worthley, Richard K Le Leu, Vicki L Whitehall, Michael Conlon, Claus Christophersen, Damien Belobrajdic, Kylie-Ann Mallitt, Ying Hu, Natsumi Irahara, Shuji Ogino, Barbara A Leggett, and Graeme P Young
Abstract: BACKGROUND: Diet is an important factor in colorectal carcinogenesis; thus, dietary supplements may have a role in colorectal cancer prevention. OBJECTIVE: The objective was to establish the relative luminal, epithelial, and epigenetic consequences of prebiotic, probiotic, and synbiotic dietary supplementation in humans. DESIGN: This was a randomized, double-blind, placebo-controlled, 4-wk crossover trial of resistant starch and Bifidobacterium lactis, either alone or as a combined synbiotic preparation, in 20 human volunteers. Rectal biopsy, feces, and serum samples were collected. The rectal mucosal endpoints were DNA methylation at 16 CpG island loci and LINE-1, epithelial proliferation (Ki67 immunohistochemistry), and crypt cellularity. The fecal endpoints were short-chain fatty acid concentrations, pH, ammonia, and microbiological profiles (by denaturing gradient gel electrophoresis and sequencing). Serum endpoints were a panel of cytokines and high-sensitivity C-reactive protein. RESULTS: Seventeen subjects completed the entire study. The synbiotic intervention fostered a significantly different fecal stream bacterial community than did either the prebiotic (P = 0.032) or the probiotic (P = 0.001) intervention alone, in part because of a greater proportion of patients harboring fecal Lachnospiraceae spp. These changes developed in the absence of any significant differences in fecal chemistry. There were no differences in epithelial kinetics. CONCLUSIONS: This synbiotic supplementation with B. lactis and resistant starch, in the doses used, induced unique changes in fecal microflora but did not significantly alter any other fecal, serum, or epithelial variables. This trial was registered in the Australian New Zealand Clinical Trials Registry at as ACTRN012606000115538.
Keywords: Intestinal Mucosa; Epithelium; Feces; Humans; Bacteria; Bifidobacterium; Colorectal Neoplasms; Inflammation; Starch; Amylose; Fatty Acids, Volatile; Drug Therapy, Combination; Cross-Over Studies; Double-Blind Method; DNA Methylation; Epigenesis, Genetic; Dietary Supplements; Probiotics; Aged; Middle Aged; Biomarkers
Rights: © 2009 American Society for Nutrition
RMID: 0020138958
DOI: 10.3945/ajcn.2009.28106
Appears in Collections:Medicine publications

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