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https://hdl.handle.net/2440/8450
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Lopata, A. | - |
dc.contributor.author | Berka, J. | - |
dc.contributor.author | Simula, A. | - |
dc.contributor.author | Norman, R. | - |
dc.contributor.author | Otani, T. | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Placenta, 1995; 16(4):335-346 | - |
dc.identifier.issn | 0143-4004 | - |
dc.identifier.issn | 1532-3102 | - |
dc.identifier.uri | http://hdl.handle.net/2440/8450 | - |
dc.description.abstract | We studied the expression of mRNA encoding the alpha- and beta-subunits of marmoset chorionic gonadotrophin (mCG) in implantation stage blastocysts and in a trophoblastic cell line derived from such blastocysts. In this investigation in situ hybridization was carried out using digoxygenin-labelled riboprobes to localize the subunit transcripts. The trophoblastic cell line, known to secrete bioactive mCG, was used as a positive control. Marmoset uterine embryos were cultured to hatched blastocysts and following growth on Matrigel or plastic were processed for in situ hybridization at developmental stages ranging from 13-15 days post-conception. In serial sections mCG-beta mRNA was detected mainly in polar trophoblast. The mRNA for mCG-alpha was expressed more uniformly in polar and mural trophoblast. Transcripts for the beta-subunit were not expressed, or present as weak signals, in the inner cell mass (ICM) and endoderm. However, low levels of mRNA for mCG-alpha were detected in the ICM and visceral endoderm. We have concluded that mRNA for mCG-beta was primarily localized to patches of syncytiotrophoblast at the embryonic pole and sparsely distributed in mural trophoblast, while the transcripts for mCG-alpha were distributed more uniformly in differentiating cytotrophoblast and syncytium, and at much lower levels in ICM and early endoderm. | - |
dc.description.statementofresponsibility | Lopata, A. ; Berka, J. ; Simula, A. ; Norman, R. ; Otani, T. | - |
dc.language.iso | en | - |
dc.publisher | W.B. Saunders | - |
dc.source.uri | http://dx.doi.org/10.1016/0143-4004(95)90091-8 | - |
dc.subject | Cells, Cultured | - |
dc.subject | Cell Line | - |
dc.subject | Trophoblasts | - |
dc.subject | Blastocyst | - |
dc.subject | Animals | - |
dc.subject | Callithrix | - |
dc.subject | Chorionic Gonadotropin | - |
dc.subject | RNA Probes | - |
dc.subject | RNA, Messenger | - |
dc.subject | In Situ Hybridization | - |
dc.subject | Transcription, Genetic | - |
dc.subject | Embryonic and Fetal Development | - |
dc.subject | Embryo Implantation | - |
dc.subject | Pregnancy | - |
dc.subject | Female | - |
dc.subject | Male | - |
dc.title | Differential distribution of mRNA for the α- and β-subunits of chorionic gonadotrophin in the implantation stage blastocyst of the marmoset monkey | - |
dc.title.alternative | Differential distribution of mRNA for the alpha- and beta-subunits of chorionic gonadotrophin in the implantation stage blastocyst of the marmoset monkey | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1016/0143-4004(95)90091-8 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Norman, R. [0000-0002-3118-3896] | - |
Appears in Collections: | Aurora harvest Obstetrics and Gynaecology publications |
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