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Type: Journal article
Title: Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Author: Cardamoni Diniz, L.
de Fatima Ruas, C.
de Paula Carvalho, V.
Torres, F.
Ruas, E.
de Oliveira Santos Garcia, M.
Sera, T.
Ruas, P.
Citation: Brazilian Archives of Biology and Technology, 2005; 48(4):511-521
Publisher: Instituto de Tecnologia do Paraná (Tecpar)
Issue Date: 2005
ISSN: 0365-0979
Statement of
Leandro Eugênio Cardamoni Diniz, Claudete de Fátima Ruas, Valdemar de Paula Carvalho, Fabrício Medeiros Torres, Eduardo Augusto Ruas, Melissa de Oliveira Santos, Tumoru Sera and Paulo Maurício Ruas
Abstract: The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.
Keywords: Coffea arabica; genetic variability; RAPD; restriction endonucleases
Rights: All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
RMID: 0020111886
DOI: 10.1590/S1516-89132005000500002
Appears in Collections:Agriculture, Food and Wine publications

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