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Type: Journal article
Title: Composition and morphology of the follicular basal lamina during atresia of bovine antral follicles
Author: Irving-Rodgers, H.
Mussard, M.
Kinder, J.
Rodgers, R.
Citation: Reproduction, 2002; 123(1):97-106
Publisher: Journals of Reproduction Fertility Ltd
Issue Date: 2002
ISSN: 1470-1626
Statement of
HF Irving-Rodgers, ML Mussard, JE Kinder, and RJ Rodgers
Abstract: The fate of the follicular basal lamina during atresia was investigated using bovine follicles, in which different follicle phenotypes have been observed. These phenotypes include: healthy follicles with rounded basal granulosa cells with an aligned basal lamina or follicles with columnar basal granulosa cells with a basal lamina of many loops (loopy), and atretic follicles in which either the antral granulosa cells (antral atresia) or the basal cells (basal atresia) die first. Loopy lamina and basal atresia occur only in small antral follicles < 5 mm in diameter. Follicles were collected from cattle of unknown reproductive history and processed for immunohistochemistry and electron microscopy, and from animals in which follicle growth had been monitored by daily measurements of follicle diameter by ultrasonography. Electron microscopic observations of dominant follicles during the growth phase, plateau and regression showed that the basal lamina was still visible and intact upon atresia. These follicles had a conventional aligned basal lamina, which they retained, except for some degree of folding, as they progressed into antral atresia. In small follicles (2-5 mm in diameter), the basal cell shape (rounded or columnar) and appearance of the basal lamina (aligned or of many loops) did not appear to be related to the type of atresia. On atresia the follicular basal laminae retained immunoreactive laminin alpha1 and beta2, type IV collagen alpha1 and nidogen. Laminin alpha2, which may come from the theca, was present in the follicular basal lamina of only 22% of healthy follicles, but was expressed very commonly in 71% of the atretic follicles. Laminin alpha2 expression was found in both phenotypes of healthy follicles, antral and basal atretic follicles, and follicles with aligned or loopy basal laminae. It is concluded that the basal lamina is not degraded upon atresia, but does undergo a variety of other changes.
Keywords: Ovarian Follicle; Basement Membrane; Animals; Cattle; Laminin; Collagen Type IV; Microscopy, Electron; Immunohistochemistry; Follicular Atresia; Female
Description: Copyright © 2002 Society for Reproduction and Fertility
RMID: 0020020211
DOI: 10.1530/rep.0.1230097
Published version:
Appears in Collections:Obstetrics and Gynaecology publications

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