Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/84783
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Type: Journal article
Title: Developing rice with high yield under phosphorus deficiency: Pup1 sequence to application
Author: Chin, J.
Gamuyao, R.
Dalid, C.
Bustamam, M.
Prasetiyono, J.
Moeljopawiro, S.
Wissuwa, M.
Heuer, S.
Citation: Plant Physiology, 2011; 156(3):1202-1216
Publisher: American Society of Plant Biologists
Issue Date: 2011
ISSN: 0032-0889
1532-2548
Statement of
Responsibility: 
Joong Hyoun Chin, Rico Gamuyao, Cheryl Dalid, Masdiar Bustamam, Joko Prasetiyono, Sugiono Moeljopawiro, Matthias Wissuwa, and Sigrid Heuer
Abstract: The major quantitative trait locus (QTL) Phosphorus uptake1 (Pup1) confers tolerance of phosphorus deficiency in soil and is currently one of the most promising QTLs for the development of tolerant rice (Oryza sativa) varieties. To facilitate targeted introgression of Pup1 into intolerant varieties, the gene models predicted in the Pup1 region in the donor variety Kasalath were used to develop gene-based molecular markers that are evenly distributed over the fine-mapped 278-kb QTL region. To validate the gene models and optimize the markers, gene expression analyses and partial allelic sequencing were conducted. The markers were tested in more than 80 diverse rice accessions revealing three main groups with different Pup1 allele constitution. Accessions with tolerant (group I) and intolerant (group III) Pup1 alleles were distinguished from genotypes with Kasalath alleles at some of the analyzed loci (partial Pup1; group II). A germplasm survey additionally confirmed earlier data showing that Pup1 is largely absent from irrigated rice varieties but conserved in varieties and breeding lines adapted to drought-prone environments. A core set of Pup1 markers has been defined, and sequence polymorphisms suitable for single-nucleotide polymorphism marker development for high-throughput genotyping were identified. Following a marker-assisted backcrossing approach, Pup1 was introgressed into two irrigated rice varieties and three Indonesian upland varieties. First phenotypic evaluations of the introgression lines suggest that Pup1 is effective in different genetic backgrounds and environments and that it has the potential to significantly enhance grain yield under field conditions.
Keywords: Oryza sativa; Seeds; Phosphorus; Genetic Markers; Reverse Transcriptase Polymerase Chain Reaction; Breeding; Gene Expression Regulation, Plant; Base Sequence; Sequence Homology, Nucleic Acid; Haplotypes; Phenotype; Genes, Plant; Quantitative Trait Loci; Models, Genetic; Molecular Sequence Data
Rights: Copyright © 2011 American Society of Plant Biologists. All rights reserved
RMID: 0020134093
DOI: 10.1104/pp.111.175471
Appears in Collections:Agriculture, Food and Wine publications

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