Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/85261
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dc.contributor.authorRobertson, S.A.-
dc.contributor.authorMayrhofer, G.-
dc.contributor.authorSeamark, R.F.-
dc.date.issued1992-
dc.identifier.citationBiology of Reproduction, 1992; 46(6):1069-1079-
dc.identifier.issn0006-3363-
dc.identifier.issn1529-7268-
dc.identifier.urihttp://hdl.handle.net/2440/85261-
dc.description.abstractCytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23,000 and 23,000-26,000, respectively. Bioassay of luminal fluids and culture supernatants were negative for the cytokines interleukin-1, interleukin-2, interleukin-3, and tumor necrosis factor-alpha. These studies identify murine uterine epithelium as a potent source of the cytokines GM-CSF and IL-6, which we postulate have potentially important functions in pregnancy through actions on target cells in both the uterus and the conceptus.-
dc.description.statementofresponsibilitySarah A. Robertson, Graham Mayrhofer, and Robert F. Seamark-
dc.language.isoen-
dc.publisherSociety for the Study of Reproduction-
dc.rightsCopyright status unknown-
dc.source.urihttp://dx.doi.org/10.1095/biolreprod46.6.1069-
dc.subjectUterus-
dc.subjectEndometrium-
dc.subjectEpithelium-
dc.subjectCells, Cultured-
dc.subjectAnimals-
dc.subjectMice, Inbred BALB C-
dc.subjectMice, Inbred C57BL-
dc.subjectMice, Inbred CBA-
dc.subjectMice-
dc.subjectMice, Nude-
dc.subjectMice, SCID-
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subjectInterleukin-6-
dc.subjectCulture Media-
dc.subjectImmunohistochemistry-
dc.subjectEstrus-
dc.subjectPregnancy-
dc.subjectPregnancy, Animal-
dc.subjectMolecular Weight-
dc.subjectFemale-
dc.titleUterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice-
dc.typeJournal article-
dc.identifier.doi10.1095/biolreprod46.6.1069-
pubs.publication-statusPublished-
dc.identifier.orcidRobertson, S.A. [0000-0002-9967-0084]-
Appears in Collections:Aurora harvest 7
Obstetrics and Gynaecology publications

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