Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/85554
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Type: Journal article
Title: Altered immunity and dendritic cell activity in the periphery of mice after long-term engraftment with bone marrow from ultraviolet-irradiated mice
Author: Ng, R.
Scott, N.
Strickland, D.
Gorman, S.
Grimbaldeston, M.
Norval, M.
Waithman, J.
Hart, P.
Citation: Journal of Immunology, 2013; 190(11):5471-5484
Publisher: American Association of Immunologists
Issue Date: 2013
ISSN: 0022-1767
1550-6606
Statement of
Responsibility: 
Royce L. X. Ng, Naomi M. Scott, Deborah H. Strickland, Shelley Gorman, Michele A. Grimbaldeston, Mary Norval, Jason Waithman and Prue H. Hart
Abstract: Alterations to dendritic cell (DC) progenitors in the bone marrow (BM) may contribute to long-lasting systemic immunosuppression (>28 d) following exposure of the skin of mice to erythemal UV radiation (UVR). DCs differentiated in vitro from the BM of mice 3 d after UVR (8 kJ/m2) have a reduced capacity to initiate immunity (both skin and airways) when adoptively transferred into naive mice. Studies in IL-10−/− mice suggested that UV-induced IL-10 was not significantly involved. To investigate the immune capabilities of peripheral tissue DCs generated in vivo from the BM of UV-irradiated mice, chimeric mice were established. Sixteen weeks after reconstitution, contact hypersensitivity responses were significantly reduced in mice reconstituted with BM from UV-irradiated mice (UV-chimeric). When the dorsal skin of UV-chimeric mice was challenged with innate inflammatory agents, the hypertrophy induced in the draining lymph nodes was minimal and significantly less than that measured in control-chimeric mice challenged with the same inflammatory agent. When DCs were differentiated from the BM of UV-chimeric mice using FLT3 ligand or GM-CSF + IL-4, the cells maintained a reduced priming ability. The diminished responses in UV-chimeric mice were not due to different numerical or proportional reconstitution of BM or the hematopoietic cells in blood, lymph nodes, and skin. Erythemal UVR may imprint a long-lasting epigenetic effect on DC progenitors in the BM and alter the function of their terminally differentiated progeny.
Keywords: Lymph Nodes
Dendritic Cells
T-Lymphocytes
Bone Marrow Cells
Animals
Mice
Dermatitis, Contact
Hypertrophy
Granulocyte-Macrophage Colony-Stimulating Factor
Membrane Proteins
Interleukin-4
Adoptive Transfer
Bone Marrow Transplantation
Ultraviolet Rays
Cell Differentiation
Cell Movement
Graft Survival
Chimerism
Female
Immunity, Innate
Rights: © 2013 by The American Association of Immunologists, Inc.
DOI: 10.4049/jimmunol.1202786
Grant ID: 1011203
539800
Appears in Collections:Aurora harvest 7
Molecular and Biomedical Science publications

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