Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/85673
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Type: Journal article
Title: Development of 15 microsatellite loci from mulloway, Argyrosomus japonicus (Pisces: Sciaenidae) using next generation sequencing and an assessment of their cross amplification in other sciaenids
Author: Barnes, T.
Izzo, C.
Bertozzi, T.
Saint, K.
Donnellan, S.
Hammer, M.
Gillanders, B.
Citation: Conservation Genetics Resources, 2014; 6(2):345-348
Publisher: Springer Netherlands
Issue Date: 2014
ISSN: 1877-7252
1877-7260
Statement of
Responsibility: 
Thomas C. Barnes, Christopher Izzo, Terry Bertozzi, Kathleen M. Saint • Stephen Donnellan, Michael P. Hammer, Bronwyn M. Gillanders
Abstract: Next generation sequencing was used to develop 15 new polymorphic microsatellite markers for the commercially and recreationally important fish mulloway (Argyrosomus japonicus). Improved knowledge of the genetic structure of the species is necessary for fisheries and environmental management. In a population of mulloway, we found that genetic variation and heterozygosity were high in most of our loci (mean number of alleles per locus = 9.46; mean heterozygosity = 0.660). To test the usefulness of our new markers for genetic research on other sciaenids, we cross amplified samples from eight other related sciaenid species, including three that form important fisheries and others that have significant conservation issues. We found that most of our new primers pairs produced scorable profiles in each of the other important sciaenids.
Keywords: Microsatellite development; Next generation DNA sequencing; Cross amplification; Argyrosomus japonicus; Kob and mulloway; Sciaenidae
Rights: © Springer Science + Business Media Dordrecht 2013
RMID: 0020137148
DOI: 10.1007/s12686-013-0090-7
Grant ID: http://purl.org/au-research/grants/arc/DP110100716
Appears in Collections:Earth and Environmental Sciences publications

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