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|Title:||A phase-segregated model for plant cell culture: the effect of cell volume fraction|
|Citation:||Journal of Chemical Engineering of Japan, 1998; 31(3):469-474|
|Publisher:||SOC CHEMICAL ENG JAPAN|
|Wei Zhang, Shintaro Furusaki, Anton P. J. Middelberg|
|Abstract:||Plant cells are characterized by low water content, so the fraction of cell volume (volume fraction) in a vessel is large compared with other cell systems, even if the cell concentrations are the same. Therefore, concentration of plant cells should preferably be expressed by the liquid volume basis rather than by the total vessel volume basis. In this paper, a new model is proposed to analyze behavior of a plant cell culture by dividing the cell suspension into the biotic- and abiotic-phases. Using this model, we analyzed the cell-growth and the alkaloid production by Catharanthus roseus. Large errors in the simulated results were observed if the phase-segregation was not considered.|
|Keywords:||Plant Cell Culture, Phase-Segregated, Modeling, Cell Volume Fraction (CVF), Structured Model|
|Rights:||© 1998 The Society of Chemical Engineers, Japan|
|Appears in Collections:||Chemical Engineering publications|
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