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Type: Journal article
Title: Membrane phospholipid asymmetry in Bacillus amyloliquefaciens
Author: Paton, J.
May, B.
Elliott, W.
Citation: Journal of Bacteriology, 1978; 135(2):393-401
Publisher: American Society for Microbiology
Issue Date: 1978
ISSN: 0021-9193
Statement of
J C Paton, B K May and W H Elliott
Abstract: The phospholipid distribution in the membrane of Bacillus amyloliquefaciens was studied by using phospholipase C (B. cereus), phospholipase A2 (Crotalus), and the nonpenetrating chemical probe trinitrobenzenesulfonic acid. After treatment of intact protoplasts of B. amyloliquefaciens with either phospholipase, about 70% of total membrane phospholipid was hydrolyzed; specifically, about 90, 90, and 30% of phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin, respectively. Under these conditions, protoplasts remained intact and sealed. However, when protoplasts that were permeabilized by cold-shock treatment were incubated with either of the phospholipases, up to 80% of cardiolipin was hydrolyzed and phosphatidylglycerol and phosphatidylethanolamine were hydrolyzed virtually to completion. In intact cells, 92% of the phosphatidylethanolamine could be labeled with trinitrobenzenesulfonic acid under conditions in which the reagent did not penetrate the membrane to any significant extent. These results indicate that 70% of total phospholipid of this bacillus exists in the outer half of the bilayer. The distribution of phosphatidylethanolamine in this bilayer is highly asymmetric with it being located predominantly in the outer half. The results with phospholipases suggest that the distributions of cardiolipin and phosphatidylglycerol are also asymmetric but independent confirmation of this is required.
Keywords: Cell Membrane
Trinitrobenzenesulfonic Acid
Membrane Lipids
Rights: Copyright © 1978 American Society for Microbiology
DOI: 10.1128/JB.135.2.393-401.1978
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Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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