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Type: Journal article
Title: Lanthanide-containing polymer microspheres by multiple-stage dispersion polymerization for highly multiplexed bioassays
Author: Abdelrahman, A.
Dai, S.
Thickett, S.
Ornatsky, O.
Bandura, D.
Baranov, V.
Winnik, M.
Citation: Journal of the American Chemical Society, 2009; 131(42):15276-15283
Publisher: American Chemical Society
Issue Date: 2009
ISSN: 0002-7863
Statement of
Ahmed I. Abdelrahman, Sheng Dai, Stuart C. Thickett, Olga Ornatsky, Dmitry Bandura, Vladimir Baranov and Mitchell A. Winnik
Abstract: We describe the synthesis and characterization of metal-encoded polystyrene microspheres by multiple-stage dispersion polymerization with diameters on the order of 2 mum and a very narrow size distribution. Different lanthanides were loaded into these microspheres through the addition of a mixture of lanthanide salts (LnCl(3)) and excess acrylic acid (AA) or acetoacetylethyl methacrylate (AAEM) dissolved in ethanol to the reaction after about 10% conversion of styrene, that is, well after the particle nucleation stage was complete. Individual microspheres contain ca. 10(6)-10(8) chelated lanthanide ions, of either a single element or a mixture of elements. These microspheres were characterized one-by-one utilizing a novel mass cytometer with an inductively coupled plasma (ICP) ionization source and time-of-flight (TOF) mass spectrometry detection. Microspheres containing a range of different metals at different levels of concentration were synthesized to meet the requirements of binary encoding and enumeration encoding protocols. With four different metals at five levels of concentration, we could achieve a variability of 624, and the strategy we report should allow one to obtain much larger variability. To demonstrate the usefulness of element-encoded beads for highly multiplexed immunoassays, we carried out a proof-of-principle model bioassay involving conjugation of mouse IgG to the surface of La and Tm containing particles and its detection by an antimouse IgG bearing a metal-chelating polymer with Pr.
Keywords: Animals
Lanthanoid Series Elements
Immunoglobulin G
Chelating Agents
Microscopy, Electron, Scanning
Molecular Structure
Surface Properties
Rights: © 2009 American Chemical Society
DOI: 10.1021/ja9052009
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Chemical Engineering publications

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