Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/86165
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Type: Journal article
Title: DNA methylation in the rectal mucosa is associated with crypt proliferation and fecal short-chain fatty acids
Author: Worthley, D.
Whitehall, V.
Le Leu, R.
Irahara, N.
Buttenshaw, R.
Mallitt, K.
Greco, S.
Ramsnes, I.
Winter, J.
Hu, Y.
Ogino, S.
Young, G.
Leggett, B.
Citation: Digestive Diseases and Sciences, 2011; 56(2):387-396
Publisher: Springer
Issue Date: 2011
ISSN: 0163-2116
1573-2568
Statement of
Responsibility: 
Daniel L. Worthley, Vicki L. J. Whitehall, Richard K. Le Leu, Natsumi Irahara, Ronald L. Buttenshaw, Kylie-Ann Mallitt, Sonia A. Greco, Ingunn Ramsnes, Jean Winter, Ying Hu, Shuji Ogino, Graeme P. Young, Barbara A. Leggett
Abstract: BACKGROUND DNA methylation varies throughout the normal colorectal mucosa and DNA methylation in normal appearing mucosa is associated with serrated and adenomatous neoplasia elsewhere within the colorectum. AIMS The purpose of this study was to measure luminal chemistry, rectal proliferation and mucosal DNA methylation and thus determine whether regional and pathological patterns of DNA methylation could be explained by luminal and epithelial factors. METHODS Twenty healthy subjects had normal rectal mucosal biopsies and a 24-h fecal collection. Rectal biopsies were analyzed for epithelial proliferation (Ki67 immunohistochemistry) and DNA methylation at 17 different markers, including “type A” markers (ESR1, GATA5, HIC1, HPP1, SFRP1), “type C” markers (MGMT, MLH1, CDKN2A, MINT1, MINT2, MINT31, IGF2, CACNA1G, NEUROG1, SOCS1, RUNX3), and LINE-1. Fecal analysis included short-chain fatty acids (SCFA), pH and ammonia. Mean “type A” and CIMP panel methylation Z-scores were calculated. RESULTS Rectal proliferation was significantly correlated with methylation at ESR1 (ρ = 0.81, P = 0.003) and GATA5 (ρ = 0.78, P = 0.012). LINE-1 methylation was 71.7 vs. 74.1%, in patients with “low” and “high” fecal total SCFA concentration (defined by the median value), respectively (P = 0.0019). On multivariate linear regression “type A” methylation was independently associated with rectal proliferation (P = 0.001). LINE-1 methylation was directly associated with rectal proliferation (P = 0.038) and total fecal SCFA concentration (P = 0.002), and inversely associated with fecal NH3 concentrations (P = 0.003). CONCLUSIONS DNA methylation in normal rectal mucosa is associated with crypt proliferation and fecal SCFA concentration. These associations may help to explain regional differences in DNA methylation as well as providing a possible link between the colorectal lumen and carcinogenesis.
Keywords: DNA methylation; Epigenetics; Colorectal cancer; Carcinogenesis
Rights: © Springer Science+Business Media, LLC 2010
RMID: 0020138943
DOI: 10.1007/s10620-010-1312-4
Grant ID: http://purl.org/au-research/grants/nhmrc/442965
Appears in Collections:Medicine publications

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