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|Title:||Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia|
|Citation:||Conservation Genetics Resources, 2014; 6(1):143-146|
|Lei Tang, Carolyn Smith-Keune, Michael G. Gardner, Britta Denise Hardesty|
|Abstract:||The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.|
|Keywords:||Black-throated Finch; Poephila cincta; Microsatellite; Conservation genetics; Next generation sequencing|
|Rights:||© Springer Science+Business Media Dordrecht 2013|
|Appears in Collections:||Aurora harvest 2|
Earth and Environmental Sciences publications
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