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Type: Journal article
Title: Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia
Author: Tang, L.
Smith-Keune, C.
Gardner, M.
Hardesty, B.
Citation: Conservation Genetics Resources, 2014; 6(1):143-146
Publisher: Springer Netherlands
Issue Date: 2014
ISSN: 1877-7252
Statement of
Lei Tang, Carolyn Smith-Keune, Michael G. Gardner, Britta Denise Hardesty
Abstract: The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.
Keywords: Black-throated Finch; Poephila cincta; Microsatellite; Conservation genetics; Next generation sequencing
Rights: © Springer Science+Business Media Dordrecht 2013
DOI: 10.1007/s12686-013-0028-0
Appears in Collections:Aurora harvest 2
Earth and Environmental Sciences publications

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