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|Title:||Production of a monoclonal antibody to human interferon-α (IFN-α) and its use to identify IFN-α-producing cells in virus infection in vivo|
|Other Titles:||Production of a monoclonal antibody to human interferon-alpha (IFN-alpha) and its use to identify IFN-alpha-producing cells in virus infection in vivo|
|Citation:||Microbial Pathogenesis, 1986; 1(2):159-168|
|Allison R. Jilbert, Paul J. Hertzog, Christopher J. Burrell, Eric J. Gowans, Anthony W. Linnane, Barrie P. Marmion|
|Abstract:||A monoclonal antibody to human interferon-alpha (IFN-alpha) was produced using affinity-purified IFN-alpha, that reacted with recombinant human IFN-alpha 2, but not with IFN-alpha 1, IFN-alpha M1 or IFN-beta. Indirect immunofluorescence using this monoclonal (designated 6C3) and anti-IFN-alpha polyclonal antibodies identified cells expressing IFN-alpha. After Sendai virus induction of normal human buffy-coat cells the proportion of monocytes and lymphocytes expressing IFN-alpha rose progressively from 0% to 50% and 34% respectively, preceding peak IFN-alpha titres in the culture supernatants. Around 80-90% of polymorphs were IFN-alpha-positive using both antisera, with or without IFN induction, although very little IFN bioactivity was released to the supernatant of polymorph cultures after IFN induction. Sections of hepatitis B virus infected human liver tissue showed foci of IFN-alpha-positive infiltrating mononuclear cells and (to a lesser extent) fibroblasts in patients who had active cirrhosis and evidence of virus replication. These findings suggest that polymorphs constitutively express IFN-alpha 2 related antigenic activity, whose biological activity is at present unknown; and demonstrates the identification of IFN-alpha-expressing cells in sections of tissue undergoing natural virus infection.|
|Keywords:||human interferon-α; monoclonal antibodies; immunofluorescence; hepatitis B virus infection|
|Rights:||©1986 Academic Press Inc. (London) Ltd.|
|Appears in Collections:||Aurora harvest 7|
Molecular and Biomedical Science publications
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