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Type: Journal article
Title: Development of wheat-Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S#1 chromosome
Author: Mago, R.
Verlin, D.
Zhang, P.
Bansal, U.
Bariana, H.
Jin, Y.
Ellis, J.
Hoxha, S.
Dundas, I.
Citation: Theoretical and Applied Genetics: international journal of plant breeding research, 2013; 126(12):2943-2955
Publisher: Springer-Verlag
Issue Date: 2013
ISSN: 0040-5752
Statement of
Rohit Mago, Dawn Verlin, Peng Zhang, Urmil Bansal, Harbans Bariana, Yue Jin, Jeffrey Ellis, Sami Hoxha, Ian Dundas
Abstract: Wild relatives of wheat are important resources for new rust resistance genes but underutilized because the valuable resistances are often linked to negative traits that prevent deployment of these genes in commercial wheats. Here, we report ph1b-induced recombinants with reduced alien chromatin derived from E.R. Sears’ wheat–Aegilops speltoides 2D-2S#1 translocation line C82.2, which carries the widely effective stem rust resistance gene Sr32. Infection type assessments of the recombinants showed that the original translocation in fact carries two stem rust resistance genes, Sr32 on the short arm and a previously undescribed gene SrAes1t on the long arm of chromosome 2S#1. Recombinants with substantially shortened alien chromatin were produced for both genes, which confer resistance to stem rust races in the TTKSK (Ug99) lineage and representative races of all Australian stem rust lineages. Selected recombinants were back crossed into adapted Australian cultivars and PCR markers were developed to facilitate the incorporation of these genes into future wheat varieties. Our recombinants and those from several other labs now show that Sr32, Sr39, and SrAes7t on the short arm and Sr47 and SrAes1t on the long arm of 2S#1 form two linkage groups and at present no rust races are described that can distinguish these resistance specificities.
Keywords: Chromosomes, Plant
Plant Stems
Genetic Markers
In Situ Hybridization, Fluorescence
Polymerase Chain Reaction
Plant Diseases
Genes, Plant
Homologous Recombination
Rights: © Springer-Verlag Berlin Heidelberg 2013
DOI: 10.1007/s00122-013-2184-8
Appears in Collections:Agriculture, Food and Wine publications
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