Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/87178
Citations
Scopus Web of Science® Altmetric
?
?
Full metadata record
DC FieldValueLanguage
dc.contributor.authorCheah, K.-
dc.contributor.authorHowarth, G.-
dc.contributor.authorBastian, S.-
dc.contributor.editorMuders, M.-
dc.date.issued2014-
dc.identifier.citationPLoS One, 2014; 9(1):e85184-1-e85184-11-
dc.identifier.issn1932-6203-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/2440/87178-
dc.description.abstractOBJECTIVE: Mucositis is a serious disorder of the gastrointestinal tract that results from cancer chemotherapy. We investigated the effects of increasing grape seed extract doses on the severity of chemotherapy in a rat model and its coincident impact on chemotherapeutic effectiveness in colon cancer cells. DESIGN: Female Dark Agouti rats were gavaged with grape seed extract (400-1000 mg/kg) or water (day 3-11) and were injected intraperitoneally with 5-Fluorouracil (150 mg/kg) or saline (control) on day 9 to induce mucositis. Daily metabolic data were collected and rats were sacrificed on day 12. Intestinal tissues were collected for histological and myeloperoxidase analyses. Caco-2 cell viability was examined in response to grape seed extract in combination with 5-Fluorouracil by 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide) assay. RESULTS: Compared with 5-Fluorouracil controls, grape seed extract (400-1000 mg/kg) significantly decreased the histological damage score (P<0.05) in the jejunum. Grape seed extract (1000 mg/kg) increased jejunal crypt depth by 25% (P<0.05) in 5-Fluorouracil treated rats compared to 5-Fluorouracil controls, and attenuated the 5-Fluorouracil -induced reduction of mucosal thickness (25%, P<0.05). Grape seed extract (600 mg/kg) decreased myeloperoxidase activity by 55% (P<0.01) compared to 5-Fluorouracil controls. Grape seed extract was more effective at ameliorating 5-Fluorouracil induced intestinal injury, with effects most pronounced in the proximal jejunum. Grape seed extract (10-25 ug/mL) significantly enhanced the growth-inhibitory effects of 5-Fluorouracil by 26% (P<0.05) in Caco-2 cells and was more potent than 5-Fluorouracil at 50-100 µg/mL. CONCLUSION: Grape seed extract may represent a new therapeutic option to decrease the symptoms of intestinal mucositis while concurrently impacting on the viability of colon cancer cells.-
dc.description.statementofresponsibilityKer Yeaw Cheah, Gordon Stanley Howarth, Susan Elaine Putnam Bastian-
dc.language.isoen-
dc.publisherPublic Library of Science-
dc.rights© 2014 Cheah et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.-
dc.subjectIntestinal Mucosa-
dc.subjectJejunum-
dc.subjectCaco-2 Cells-
dc.subjectAnimals-
dc.subjectHumans-
dc.subjectRats-
dc.subjectFluorouracil-
dc.subjectPeroxidase-
dc.subjectAntioxidants-
dc.subjectAdministration, Oral-
dc.subjectInjections, Intraperitoneal-
dc.subjectSeverity of Illness Index-
dc.subjectCell Proliferation-
dc.subjectCell Survival-
dc.subjectFemale-
dc.subjectMucositis-
dc.subjectGrape Seed Extract-
dc.titleGrape seed extract dose-responsively decreases disease severity in a rat model of mucositis; concomitantly enhancing chemotherapeutic effectiveness in colon cancer cells-
dc.typeJournal article-
dc.identifier.doi10.1371/journal.pone.0085184-
pubs.publication-statusPublished-
dc.identifier.orcidHowarth, G. [0000-0001-6979-6084]-
dc.identifier.orcidBastian, S. [0000-0002-8790-2044]-
Appears in Collections:Animal and Veterinary Sciences publications
Aurora harvest 2

Files in This Item:
File Description SizeFormat 
hdl_87178.pdfPublished version1.18 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.