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|Title:||Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)|
Van Dijk, J.K.
|Citation:||American Journal of Botany, 2011; 98(4):e81-e83|
|Publisher:||Botanical Society of America|
|Michelle Waycott, Brendan L. Jones, Jent Kornelis Van Dijk, Heather L. A. Robson and Ainsley Calladine|
|Abstract:||Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.|
|Keywords:||microsatellite; polyploidy; Solanum centrale|
|Rights:||© 2011 Botanical Society of America|
|Appears in Collections:||Ecology, Evolution and Landscape Science publications|
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