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Type: Journal article
Title: Microsatellite markers in the Australian desert plant, Solanum centrale (Solanaceae)
Author: Waycott, M.
Jones, B.L.
Van Dijk, J.K.
Robson, H.L.
Calladine, A.
Citation: American Journal of Botany, 2011; 98(4):e81-e83
Publisher: Botanical Society of America
Issue Date: 2011
ISSN: 0002-9122
Statement of
Michelle Waycott, Brendan L. Jones, Jent Kornelis Van Dijk, Heather L. A. Robson and Ainsley Calladine
Abstract: Premise of the study: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. Methods and Results: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. Conclusions: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.
Keywords: microsatellite; polyploidy; Solanum centrale
Rights: © 2011 Botanical Society of America
RMID: 0030009141
DOI: 10.3732/ajb.1000356
Appears in Collections:Ecology, Evolution and Landscape Science publications

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