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Type: Journal article
Title: Proteomics profiles from mass spectrometry
Author: Koch, I.
Hoffmann, P.
Marron, J.
Citation: Electronic Journal of Statistics, 2014; 8(2):1703-1713
Publisher: Institute of Mathematical Statistics
Issue Date: 2014
ISSN: 1935-7524
Statement of
Inge Koch, Peter Hoffmann and J. S. Marron
Abstract: Proteomics is a rapidly growing research area within bioinformatics which focuses on quantification of peptide concentrations and on the identification of proteins and peptides. In quantitative proteomics the identification of biomarkers from peptide concentrations is important for diagnostic purposes and treatment of diseases. The goal of this paper is to facilitate research in this area, by providing a test bed for comparison of 1D curve registration methods. This is done in a novel way, by providing not only curves, but also an answer key as to how the peaks should align. In the following papers a number of approaches to this problem are given, and the answer key provides unusually useful insights into how the methods compare. For this reason, we consider proteomics mass spectrometry profiles which are part of a larger study into the identification of biomarkers in Acute Myeloid Leukaemia (AML). For these profiles large ion counts result in large peaks, but these peaks may occur at different retention times for different profiles. The first step in the quantification of peptides in proteomics profiles is the alignment of the 1D curves of total ion count (TIC). The paper includes a description of proteomics mass spectrometry profiling, and considers profiles from five patients with AML. It outlines the preprocessing steps we applied to the multiple TIC samples from each patient, and introduces the reference peptides. The retention times of the reference peptides are known for each profile, and using these times as an answer key makes the 1D TIC curves a particularly informative test bed for curve registration.
Keywords: Mass spectrometry; one-dimensional curve reg- istration; peak alignment; proteomics; total ion counts
Rights: Copyright status unknown
RMID: 0030015603
DOI: 10.1214/14-EJS900
Appears in Collections:Molecular and Biomedical Science publications

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