Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/88172
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Alterations in the mitochondrial alternative NAD(P)H dehydrogenase NDB4 lead to changes in mitochondrial electron transport chain composition, plant growth and response to oxidative stress
Author: Smith, C.
Barthet, M.
Melino, V.
Smith, P.
Day, D.
Soole, K.
Citation: Plant and Cell Physiology, 2011; 52(7):1222-1237
Publisher: Oxford University Press
Issue Date: 2011
ISSN: 0032-0781
1471-9053
Statement of
Responsibility: 
Chevaun Smith, Michelle Barthet, Vanessa Melino, Penelope Smith, David Day, and Kathleen Soole
Abstract: The branched respiratory electron transport chain of plants contains a non-phosphorylating alternative pathway consisting of type II NAD(P)H dehydrogenases on both sides of the inner membrane linked through the ubiquinone pool to an alternative oxidase (AOX). T-DNA and RNA interference (RNAi) were used to reduce gene expression to characterize the external NAD(P)H dehydrogenase NDB4 in Arabidopsis. The ndb4 lines showed different levels of suppression of NDB4 protein, leading to increases in NBD2 and AOX1a mRNA and protein levels in all lines. These changes were associated with lower reactive oxygen species formation and an altered phenotype, including changes in growth rate, root : shoot ratios and leaf area. The general growth pattern for the ndb4 mutants was decreased leaf area early in development (6-15 d) followed by a prompt subsequent increase in leaf area that exceeded the leaf area of the wild type by maturity (the 10-12 rosette stage). This pattern was most evident for the RNAi lines that had increased mitochondrial electron transport capacity. The RNAi lines also exhibited better tolerance to salinity stress, with better growth rates and lower shoot Na⁺ content compared with controls when grown under saline conditions. We hypothesize that these differences reflect the enhanced expression of NDB2 and AOX in the ndb4 mutant plants.
Keywords: Alternative NAD(P)H dehydrogenases; Alternative oxidase; Arabidopsis thaliana; Mitochondria; Respiration; Salinity stress
Rights: © The Author 2011
DOI: 10.1093/pcp/pcr073
Grant ID: http://purl.org/au-research/grants/arc/DP0560102
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 2

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.