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|Title:||Metal-containing polystyrene beads as standards for mass cytometry|
|Citation:||Journal of Analytical Atomic Spectrometry, 2010; 25(3):260-268|
|Publisher:||Royal Society of Chemistry|
|Ahmed I. Abdelrahman, Olga Ornatsky, Dmitry Bandura, Vladimir Baranov, Robert Kinach, Sheng Dai, Stuart C. Thickett, Scott Tanner and Mitchell A. Winnik|
|Abstract:||We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.|
|Rights:||©The Royal Society of Chemistry 2010|
|Appears in Collections:||Aurora harvest 2|
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