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|Title:||High resolution mapping of Dense spike-ar (dsp.ar) to the genetic centromere of barley chromosome 7H|
|Citation:||Theoretical and Applied Genetics, 2012; 124(2):373-384|
|Fahimeh Shahinnia, Arnis Druka, Jerome Franckowiak, Michele Morgante, Robbie Waugh and Nils Stein|
|Abstract:||Spike density in barley is under the control of several major genes, as documented previously by genetic analysis of a number of morphological mutants. One such class of mutants affects the rachis internode length leading to dense or compact spikes and the underlying genes were designated dense spike (dsp). We previously delimited two introgressed genomic segments on chromosome 3H (21 SNP loci, 35.5 cM) and 7H (17 SNP loci, 20.34 cM) in BW265, a BC(7)F(3) nearly isogenic line (NIL) of cv. Bowman as potentially containing the dense spike mutant locus dsp.ar, by genotyping 1,536 single nucleotide polymorphism (SNP) markers in both BW265 and its recurrent parent. Here, the gene was allocated by high-resolution bi-parental mapping to a 0.37 cM interval between markers SC57808 (Hv_SPL14)-CAPSK06413 residing on the short and long arm at the genetic centromere of chromosome 7H, respectively. This region putatively contains more than 800 genes as deduced by comparison with the collinear regions of barley, rice, sorghum and Brachypodium, Classical map-based isolation of the gene dsp.ar thus will be complicated due to the infavorable relationship of genetic to physical distances at the target locus.|
|Keywords:||Chromosomes, Plant; Centromere; Hordeum; Flowers; DNA Primers; Genetic Markers; Chromosome Mapping; Sequence Analysis, DNA; Base Sequence; Phenotype; Genes, Plant; Molecular Sequence Data|
|Rights:||© Springer-Verlag 2011|
|Appears in Collections:||Agriculture, Food and Wine publications|
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