Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/88410
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Type: Journal article
Title: H-NS plays a role in expression of Acinetobacter baumannii virulence features
Author: Eijkelkamp, B.
Stroeher, U.
Hassan, K.
Elbourne, L.
Paulsen, I.
Brown, M.
Citation: Infection and Immunity, 2013; 81(7):2574-2583
Publisher: American Society for Microbiology
Issue Date: 2013
ISSN: 0019-9567
1098-5522
Statement of
Responsibility: 
Bart A. Eijkelkamp, Uwe H. Stroeher, Karl A. Hassan, Liam D. H. Elbourne, Ian T. Paulsen, Melissa H. Brown
Abstract: Acinetobacter baumannii has become a major problem in the clinical setting with the prevalence of infections caused by multidrug- resistant strains on the increase. Nevertheless, only a limited number of molecular mechanisms involved in the success of A. baumannii as a human pathogen have been described. In this study, we examined the virulence features of a hypermotile derivative of A. baumannii strain ATCC 17978, which was found to display enhanced adherence to human pneumocytes and elevated levels of lethality toward Caenorhabditis elegans nematodes. Analysis of cellular lipids revealed modifications to the fatty acid composition, providing a possible explanation for the observed changes in hydrophobicity and subsequent alteration in adherence and motility. Comparison of the genome sequences of the hypermotile variant and parental strain revealed that an insertion sequence had disrupted an hns-like gene in the variant. This gene encodes a homologue of the histone-like nucleoid structuring (H-NS) protein, a known global transcriptional repressor. Transcriptome analysis identified the global effects of this mutation on gene expression, with major changes seen in the autotransporter Ata, a type VI secretion system, and a type I pilus cluster. Interestingly, isolation and analysis of a second independent hypermotile ATCC 17978 variant revealed a mutation to a residue within the DNA binding region of H-NS. Taken together, these mutants indicate that the phenotypic and transcriptomic differences seen are due to loss of regulatory control effected by H-NS.
Keywords: Cell Line, Tumor
Animals
Humans
Caenorhabditis elegans
Biofilms
Acinetobacter baumannii
Acinetobacter Infections
Fatty Acids
Bacterial Proteins
DNA-Binding Proteins
Repressor Proteins
DNA, Bacterial
DNA Transposable Elements
Genetic Complementation Test
Gene Expression Profiling
Computational Biology
Bacterial Adhesion
Virulence
Gene Expression Regulation, Bacterial
Base Composition
Binding Sites
Amino Acid Sequence
Mutation
Genes, Bacterial
Molecular Sequence Data
Hydrophobic and Hydrophilic Interactions
Bacterial Secretion Systems
Alveolar Epithelial Cells
Rights: Copyright © 2013, American Society for Microbiology. All Rights Reserved
DOI: 10.1128/IAI.00065-13
Grant ID: http://purl.org/au-research/grants/nhmrc/535053
http://purl.org/au-research/grants/arc/DP110102680
Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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