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Type: Journal article
Title: Development of a multiplex panel of microsatellite markers for two species of gliding marsupials, Petaurus breviceps and Petaurus norfolcensis
Author: Malekian, M.
Dudaniec, R.
Saint, K.
Carthew, S.
Cooper, S.
Citation: Australian Journal of Zoology, 2013; 61(6):475-478
Publisher: CSIRO
Issue Date: 2013
ISSN: 0004-959X
Statement of
M. Malekian, R.Y. Dudaniec, K.M. Saint, S.M. Carthew and S.J.B. Cooper
Abstract: Here, we describe the development of seven new microsatellite loci from Petaurus breviceps. Together with eight loci from previous studies of gliders, we tested their utility for amplification, multiplexing and polymorphism in two glider species, P. breviceps and P. norfolcensis. Of the 15 loci tested, all were polymorphic in P. breviceps and 12 were polymorphic in P. norfolcensis. Overall, 260 sugar gliders from 13 sites in south-eastern South Australia and 106 squirrel gliders collected through out south-east Queensland were used in analyses. Numbers of alleles per locus ranged from 4 to 27 in P. breviceps and from 2 to 44 in P. norfolcensis Observed heterozygosity ranged between 0.438 and 0.904 in P. breviceps and between 0.189 and 0.981 in P. norfolcensis.Within the populations analysed, one of the 15 loci for P. breviceps and two of the 12 loci for P. norfolcensis deviated from Hardy–Weinberg equilibrium. The micro satellite loci will provide valuable tools for further study of social organisation, mating systems and population biology of these gliding marsupials
Keywords: 454 sequencing; habitat fragmentation; microsatellites; population structure
Rights: © CSIRO 2013
DOI: 10.1071/ZO14002
Appears in Collections:Aurora harvest 7
Earth and Environmental Sciences publications

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