Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/88605
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dc.contributor.author | Wilson, D. | - |
dc.contributor.author | Fowkes, F. | - |
dc.contributor.author | Gilson, P. | - |
dc.contributor.author | Elliott, S. | - |
dc.contributor.author | Tavul, L. | - |
dc.contributor.author | Michon, P. | - |
dc.contributor.author | Dabod, E. | - |
dc.contributor.author | Siba, P. | - |
dc.contributor.author | Mueller, I. | - |
dc.contributor.author | Crabb, B. | - |
dc.contributor.author | Beeson, J. | - |
dc.contributor.editor | Snounou, G. | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | PLoS One, 2011; 6(11):e27705-1-e27705-14 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/88605 | - |
dc.description.abstract | Background: Antibodies targeting blood stage antigens are important in protection against malaria, but the key targets and mechanisms of immunity are not well understood. Merozoite surface protein 1 (MSP1) is an abundant and essential protein. The C-terminal 19 kDa region (MSP1-19) is regarded as a promising vaccine candidate and may also be an important target of immunity. Methodology/Findings: Growth inhibitory antibodies against asexual-stage parasites and IgG to recombinant MSP1-19 were measured in plasma samples from a longitudinal cohort of 206 children in Papua New Guinea. Differential inhibition by samples of mutant P. falciparum lines that expressed either the P. falciparum or P. chabaudi form of MSP1-19 were used to quantify MSP1-19 specific growth-inhibitory antibodies. The great majority of children had detectable IgG to MSP1-19, and high levels of IgG were significantly associated with a reduced risk of symptomatic P. falciparum malaria during the 6-month follow-up period. However, there was little evidence of PfMSP1-19 specific growth inhibition by plasma samples from children. Similar results were found when testing non-dialysed or dialysed plasma, or purified antibodies, or when measuring growth inhibition in flow cytometry or microscopy-based assays. Rabbit antisera generated by immunization with recombinant MSP1-19 demonstrated strong MSP1-19 specific growth-inhibitory activity, which appeared to be due to much higher antibody levels than human samples; antibody avidity was similar between rabbit antisera and human plasma. Conclusions/Significance: These data suggest that MSP1-19 is not a major target of growth inhibitory antibodies and that the protective effects of antibodies to MSP1-19 are not due to growth inhibitory activity, but may instead be mediated by other mechanisms. Alternatively, antibodies to MSP1-19 may act as a marker of protective immunity. | - |
dc.description.statementofresponsibility | Danny W. Wilson, Freya J. I. Fowkes, Paul R. Gilson, Salenna R. Elliott, Livingstone Tavul, Pascal Michon, Elija Dabod, Peter M. Siba, Ivo Mueller, Brendan S. Crabb, James G. Beeson | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | © 2011 Wilson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0027705 | - |
dc.subject | Animals | - |
dc.subject | Humans | - |
dc.subject | Plasmodium falciparum | - |
dc.subject | Parasitemia | - |
dc.subject | Malaria | - |
dc.subject | Recurrence | - |
dc.subject | Merozoite Surface Protein 1 | - |
dc.subject | Antibodies, Protozoan | - |
dc.subject | Vaccination | - |
dc.subject | Antibody Specificity | - |
dc.subject | Molecular Weight | - |
dc.subject | Adolescent | - |
dc.subject | Child | - |
dc.subject | Child, Preschool | - |
dc.subject | Adaptive Immunity | - |
dc.title | Quantifying the importance of MSP1-19 as a target of growth-inhibitory and protective antibodies against Plasmodium falciparum in humans | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0027705 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Wilson, D. [0000-0002-5073-1405] | - |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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hdl_88605.pdf | Published version | 840.22 kB | Adobe PDF | View/Open |
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