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Type: Journal article
Title: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo
Author: Gronthos, S.
Mankani, M.
Brahim, J.
Robey, P.G.
Shi, S.
Citation: Proceedings of the National Academy of Sciences of the United States of America, 2000; 97(25):13625-13630
Publisher: National Academy of Sciences of the United States of America
Issue Date: 2000
ISSN: 0027-8424
Statement of
S. Gronthos, M. Mankani, J. Brahim, P. Gehron Robey, and S. Shi
Abstract: Dentinal repair in the postnatal organism occurs through the activity of specialized cells, odontoblasts, that are thought to be maintained by an as yet undefined precursor population associated with pulp tissue. In this study, we isolated a clonogenic, rapidly proliferative population of cells from adult human dental pulp. These DPSCs were then compared with human bone marrow stromal cells (BMSCs), known precursors of osteoblasts. Although they share a similar immunophenotype in vitro, functional studies showed that DPSCs produced only sporadic, but densely calcified nodules, and did not form adipocytes, whereas BMSCs routinely calcified throughout the adherent cell layer with clusters of lipid-laden adipocytes. When DPSCs were transplanted into immunocompromised mice, they generated a dentin-like structure lined with human odontoblast-like cells that surrounded a pulp-like interstitial tissue. In contrast, BMSCs formed lamellar bone containing osteocytes and surface-lining osteoblasts, surrounding a fibrous vascular tissue with active hematopoiesis and adipocytes. This study isolates postnatal human DPSCs that have the ability to form a dentin/pulp-like complex.
Keywords: odontoblast; dentin; in vivo transplantation
Rights: Copyright status unknown
RMID: 0030016998
DOI: 10.1073/pnas.240309797
Appears in Collections:Medical Sciences publications

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