Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8882
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Type: Journal article
Title: Accessible xenografts of human synovium in the subcutaneous tissues of the ears of SCID mice
Author: Proudman, S.
Cleland, L.
Fusco, M.
Mayrhofer, G.
Citation: Immunology and Cell Biology, 1999; 77(2, April 1999):109-120
Publisher: WILEY
Issue Date: 1999
ISSN: 0818-9641
1440-1711
Abstract: This work was undertaken to examine whether human synovium could be engrafted into subcutaneous pouches in the ears of severe combined immunodeficient (SCID) mice. Synovium was transplanted into surgically constructed ear pouches. The grafts were examined by histological and immunohistochemical methods after varying periods after engraftment, or after percutaneous injection of TNF-alpha. Normal, osteo-arthritic and rheumatoid synovium was engrafted successfully in subcutaneous ear pouches. The general morphology and cellular compositions of xenografts were retained including human endothelial cells. In rheumatoid xenografts, macrophages, fibroblasts and lymphocytes persisted for at least 4 weeks. Vascular expression of intercellular adhesion molecule-1 (ICAM-1) was maintained but expression of vascular adhesion molecule-1 (VCAM-1), E-selectin and MHC class II diminished with time. Percutaneous injection of TNF-alpha induced up-regulation of VCAM-1. Human synovium can be engrafted into subcutaneous ear pouches in SCID mice. The xenografts are accessible and respond to injection of a pro-inflammatory cytokine.
Keywords: Synovial Membrane; Animals; Humans; Mice; Mice, SCID; Arthritis, Rheumatoid; Osteoarthritis; Tumor Necrosis Factor-alpha; Recombinant Proteins; Transplantation, Heterologous; Immunophenotyping; Graft Survival; Neovascularization, Physiologic; Adult; Middle Aged; Female; Male; Synovectomy
RMID: 0030004927
DOI: 10.1046/j.1440-1711.1999.00802.x
Appears in Collections:Medicine publications

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