Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/89171
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Type: Journal article
Title: Genotyping single nucleotide polymorphisms using different molecular beacon multiplexed within a suspended core optical fiber
Author: Linh, V.
Giannetti, S.
Warren-Smith, S.
Cooper, A.
Selleri, S.
Cucinotta, A.
Monro, T.
Citation: Sensors, 2014; 14(8):14488-14499
Publisher: MDPI AG
Issue Date: 2014
ISSN: 1424-8220
1424-8220
Statement of
Responsibility: 
Linh Viet Nguyen, Sara Giannetti, Stephen Warren-Smith, Alan Cooper, Stefano Selleri, Annamaria Cucinotta, and Tanya Monro
Abstract: We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.
Keywords: DNA; Genotype; Polymorphism, Single Nucleotide; Fluorescence; Optical Fibers; Genotyping Techniques
Rights: © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
RMID: 0030017722
DOI: 10.3390/s140814488
Appears in Collections:Ecology, Evolution and Landscape Science publications

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