Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/89177
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Type: Journal article
Title: IcsA is a Shigella flexneri adhesin regulated by the type III secretion system and required for pathogenesis
Author: Zumsteg, A.
Goosmann, C.
Brinkmann, V.
Morona, R.
Zychlinsky, A.
Citation: Cell Host and Microbe, 2014; 15(4):435-445
Publisher: Elsevier
Issue Date: 2014
ISSN: 1931-3128
1934-6069
Statement of
Responsibility: 
Anna Brotcke Zumsteg, Christian Goosmann, Volker Brinkmann, Renato Morona, Arturo Zychlinsky
Abstract: Following contact with the epithelium, the enteric intracellular bacterial pathogen Shigella flexneri invades epithelial cells and escapes intracellular phagosomal destruction using its type III secretion system (T3SS). The bacterium replicates within the host cell cytosol and spreads between cells using actin-based motility, which is mediated by the virulence factor IcsA (VirG). Whereas S. flexneri invasion is well characterized, adhesion mechanisms of the bacterium remain elusive. We found that IcsA also functions as an adhesin that is both necessary and sufficient to promote contact with host cells. As adhesion can be beneficial or deleterious depending on the host cell type, S. flexneri regulates IcsA-dependent adhesion. Activation of the T3SS in response to the bile salt deoxycholate triggers IcsA-dependent adhesion and enhances pathogen invasion. IcsA-dependent adhesion contributes to virulence in a mouse model of shigellosis, underscoring the importance of this adhesin to S. flexneri pathogenesis.
Keywords: Cell Line, Tumor; Caco-2 Cells; Hela Cells; Epithelial Cells; Animals; Humans; Mice; Shigella flexneri; Dysentery, Bacillary; Deoxycholic Acid; Bacterial Proteins; DNA-Binding Proteins; Transcription Factors; Cholagogues and Choleretics; Antigens, Bacterial; Bacterial Adhesion; Bacterial Secretion Systems
Rights: Copyright © 2014 Elsevier Inc. All rights reserved.
RMID: 0030008880
DOI: 10.1016/j.chom.2014.03.001
Appears in Collections:Molecular and Biomedical Science publications

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