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dc.contributor.authorGarrod, T.en
dc.contributor.authorGrubor-Bauk, B.en
dc.contributor.authorGargett, T.en
dc.contributor.authorLi, Y.en
dc.contributor.authorMiller, D.en
dc.contributor.authorYu, W.en
dc.contributor.authorMajor, L.en
dc.contributor.authorBurrell, C.en
dc.contributor.authorWesselingh, S.en
dc.contributor.authorSuhrbier, A.en
dc.contributor.authorGowans, E.en
dc.identifier.citationEuropean Journal of Immunology, 2014; 44(7):1992-2002en
dc.description.abstractTraditional vaccine strategies are inefficient against challenge with complex pathogens including HIV; therefore, novel vaccine technologies are required. DNA vaccines are attractive as they are relatively cheap and easy to manufacture, but a major limitation has been their lack of immunogenicity in humans, which may be overcome with the incorporation of an adjuvant. HSP70 is a recognised damage-associated molecular pattern, which is a potential adjuvant. We investigated the immunogenicity of a DNA vaccine encoding HIV gag and HSP70; the latter was genetically modified to produce cytoplasmic, secreted or membrane-bound HSP70, the expression of which was controlled by an independent promoter. The DNA was administered to C57BL/6 mice to evaluate gag-specific T-cell responses. Our results demonstrated the ability of membrane-bound and secreted HSP70 to significantly enhance gag-specific T-cell responses and increase the breadth of T-cell responses to include subdominant epitopes. Membrane-bound or secreted HSP70 also significantly improved the multifunctionality of HIV-specific T cells and T-cell proliferation, which is important for maintaining T-cell integrity. Most importantly, the inclusion of membrane-bound HSP70, secreted HSP70 or a combination significantly increased protection in mice challenged with EcoHIV, a chimeric virus that replicates in mouse leukocytes in vivo.en
dc.description.statementofresponsibilityTamsin J. Garrod, Branka Grubor-Bauk, Tessa Gargett, Yanrui Li, Darren S. Miller, Wenbo Yu, Lee Major, Christopher J. Burrell, Steven Wesselingh, Andreas Suhrbier, and Eric J. Gowansen
dc.publisherWiley-VCH Verlagen
dc.rights© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheimen
dc.subjectAdjuvant; DNA vaccines; HSP70en
dc.titleDNA vaccines encoding membrane-bound or secreted forms of heat shock protein 70 exhibit improved potencyen
dc.typeJournal articleen
dc.identifier.orcidGrubor-Bauk, B. [0000-0002-4642-105X]en
dc.identifier.orcidGargett, T. [0000-0003-3713-1373]en
dc.identifier.orcidBurrell, C. [0000-0002-4020-349X]en
dc.identifier.orcidGowans, E. [0000-0002-4274-8311]en
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