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Type: Journal article
Title: Boronate probes for the detection of hydrogen peroxide release from human spermatozoa
Author: Purdey, M.
Connaughton, H.
Whiting, S.
Schartner, E.
Monro, T.
Thompson, J.
John Aitken, R.
Abell, A.
Citation: Free Radical Biology & Medicine, 2015; 81:69-76
Publisher: Elsevier
Issue Date: 2015
ISSN: 0891-5849
Statement of
Malcolm S. Purdey, Haley S. Connaughton, Sara Whiting, Erik P. Schartner, Tanya M. Monro, Jeremy G. Thompson, R. John Aitken, Andrew D. Abell
Abstract: Human spermatozoa are compromised by production of reactive oxygen species (ROS) and detection of ROS in spermatozoa is important for the diagnosis of male infertility. Probes 2',7'-dichlorohydrofluorescein diacetate (DCFH), dihydroethidium (DHE) and MitoSOX red (MSR) are commonly used for detecting ROS by flow cytometry, however these probes lack sensitivity to hydrogen peroxide (H2O2), which is particularly damaging to mammalian sperm cells. This study reports the synthesis and use of three aryl boronate probes, peroxyfluor-1 (PF1), carboxy peroxyfluor-1 (CPF1) and a novel probe 2(2-ethoxyethoxy)ethoxy peroxyfluor-1 (EEPF1) in human spermatozoa. PF1 and EEPF1 were found to be effective in detecting H2O2 and peroxynitrite (ONOO(-)) produced by spermatozoa when stimulated with menadione or 4-hydroxynonenal. EEPF1 was more effective at detection of ROS in spermatozoa than DCFH, DHE and MSR; furthermore it distinguished poorly motile sperm as shown by greater ROS production. EEPF1 should therefore have a significant role in diagnosis of oxidative stress in male infertility, cryopreservation, age, lifestyle and exposure to environmental toxicants.
Keywords: Aryl boronate; Free radicals; Human spermatozoa; Hydrogen peroxide; Organic synthesis; Reactive oxygen species
Rights: © 2015 Elsevier Inc.
RMID: 0030021624
DOI: 10.1016/j.freeradbiomed.2015.01.015
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Appears in Collections:Paediatrics publications

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