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Type: Journal article
Title: Rice GLYCOSYLTRANSFERASE1 encodes a glycosyltransferase essential for pollen wall formation
Author: Moon, S.
Kim, S.
Zhao, G.
Yi, J.
Yoo, Y.
Jin, P.
Lee, S.
Jung, K.
Zhang, D.
An, G.
Citation: Plant Physiology, 2013; 161(2):663-675
Publisher: American Society of Plant Biologists
Issue Date: 2013
ISSN: 0032-0889
Statement of
Sunok Moon, Sung-Ryul Kim, Guochao Zhao, Jakyung Yi, Youngchul Yoo, Ping Jin, Sang-Won Lee, Ki-hong Jung, Dabing Zhang, and Gynheung An
Abstract: The pollen wall consists of an exine and an intine. The mechanism underlying its formation is not well understood. Glycosyltransferases catalyze the modification of biological molecules by attaching a single or multiple sugars and play key roles in a wide range of biological processes. We examined the role of GLYCOSYLTRANSFERASE1 (OsGT1) in pollen wall development in rice (Oryza sativa). This gene is highly expressed in mature pollen, and plants containing alleles caused by transfer DNA insertion do not produce homozygous progeny. Reciprocal crosses between OsGT1/osgt1 and the wild type indicated that the mutation leads to a male gametophyte defect. Microscopic analyses revealed that osgt1 pollen developed normally to the pollen mitosis stage but failed to produce mature grains. In osgt1 pollen, intine structure was disrupted. In addition, starch and protein levels were much lower in the mutant grains. Recombinant OsGT1 transferred glucose from UDP-glucose to the third and seventh positions of quercetin, a universal substrate of glycosyltransferases. Consistent with the role of OsGT1, an OsGT1-green fluorescent protein fusion protein was localized to the Golgi apparatus. Taken together, our results suggest that OsGT1 is a Golgi-localized glycosyltransferase essential for intine construction and pollen maturation, providing new insight into male reproductive development.
Keywords: Golgi Apparatus
Plants, Genetically Modified
Green Fluorescent Proteins
Plant Proteins
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Gene Expression Profiling
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Plant
Rights: © 2013 American Society of Plant Biologists. All Rights Reserved.
DOI: 10.1104/pp.112.210948
Appears in Collections:Agriculture, Food and Wine publications
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