Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/89632
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DC Field | Value | Language |
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dc.contributor.author | Huang, H. | - |
dc.contributor.author | Cheng, F. | - |
dc.contributor.author | Wang, R. | - |
dc.contributor.author | Zhang, D. | - |
dc.contributor.author | Yang, L. | - |
dc.contributor.editor | Forster, R. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | PLoS One, 2013; 8(9):e75850-1-e75850-9 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/89632 | - |
dc.description.abstract | Proper selection of endogenous reference genes and their real-time PCR assays is quite important in genetically modified organisms (GMOs) detection. To find a suitable endogenous reference gene and its real-time PCR assay for common wheat (Triticum aestivum L.) DNA content or copy number quantification, four previously reported wheat endogenous reference genes and their real-time PCR assays were comprehensively evaluated for the target gene sequence variation and their real-time PCR performance among 37 common wheat lines. Three SNPs were observed in the PKABA1 and ALMT1 genes, and these SNPs significantly decreased the efficiency of real-time PCR amplification. GeNorm analysis of the real-time PCR performance of each gene among common wheat lines showed that the Waxy-D1 assay had the lowest M values with the best stability among all tested lines. All results indicated that the Waxy-D1 gene and its real-time PCR assay were most suitable to be used as an endogenous reference gene for common wheat DNA content quantification. The validated Waxy-D1 gene assay will be useful in establishing accurate and creditable qualitative and quantitative PCR analysis of GM wheat. | - |
dc.description.statementofresponsibility | Huali Huang, Fang Cheng, Ruoan Wang, Dabing Zhang, Litao Yang | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | © 2013 Huang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0075850 | - |
dc.subject | Plants, Genetically Modified | - |
dc.subject | Triticum | - |
dc.subject | Starch Synthase | - |
dc.subject | Plant Proteins | - |
dc.subject | DNA Primers | - |
dc.subject | Reproducibility of Results | - |
dc.subject | Polymorphism, Single Nucleotide | - |
dc.subject | Genes, Plant | - |
dc.subject | Genetic Variation | - |
dc.subject | Real-Time Polymerase Chain Reaction | - |
dc.title | Evaluation of four endogenous reference genes and their real-time PCR assays for common wheat quantification in GMOs detection | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0075850 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Zhang, D. [0000-0003-3181-9812] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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hdl_89632.pdf | Published version | 733.75 kB | Adobe PDF | View/Open |
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