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Type: Journal article
Title: Collaborative ring trial of the papaya endogenous reference gene and its polymerase chain reaction assays for genetically modified organism analysis
Author: Wei, J.
Li, F.
Guo, J.
Li, X.
Xu, J.
Wu, G.
Zhang, D.
Yang, L.
Citation: Journal of Agricultural and Food Chemistry, 2013; 61(47):11363-11370
Publisher: American Chemical Society
Issue Date: 2013
ISSN: 0021-8561
Statement of
Jiaojun Wei, Feiwu Li, Jinchao Guo, Xiang Li, Junfeng Xu, Gang Wu, Dabing Zhang, and Litao Yang
Abstract: The papaya (Carica papaya L.) Chymopapain (CHY) gene has been reported as a suitable endogenous reference gene for genetically modified (GM) papaya detection in previous studies. Herein, we further validated the use of the CHY gene and its qualitative and quantitative polymerase chain reaction (PCR) assays through an interlaboratory collaborative ring trial. A total of 12 laboratories working on detection of genetically modified organisms participated in the ring trial and returned test results. Statistical analysis of the returned results confirmed the species specificity, low heterogeneity, and single-copy number of the CHY gene among different papaya varieties. The limit of detection of the CHY qualitative PCR assay was 0.1%, while the limit of quantification of the quantitative PCR assay was ∼25 copies of haploid papaya genome with acceptable PCR efficiency and linearity. The differences between the tested and true values of papaya content in 10 blind samples ranged from 0.84 to 6.58%. These results indicated that the CHY gene was suitable as an endogenous reference gene for the identification and quantification of GM papaya.
Keywords: collaborative ring trial; Carica papaya; Chymopapain; endogenous reference gene; qualitative and quantitative PCR
Rights: © 2013 American Chemical Society
RMID: 0030023448
DOI: 10.1021/jf403338a
Appears in Collections:Agriculture, Food and Wine publications

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