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Type: Journal article
Title: Antiviral efficacy of nine nucleoside reverse transcriptase inhibitors against feline immunodeficiency virus in feline peripheral blood mononuclear cells
Author: Schwartz, A.
McCrackin, M.
Schinazi, R.
Hill, P.
Vahlenkamp, T.
Tompkins, M.
Hartmann, K.
Citation: American Journal of Veterinary Research, 2014; 75(3):273-281
Publisher: American Veterinary Medical Association
Issue Date: 2014
ISSN: 0002-9645
Statement of
Anita M. Schwartz, Mary Ann McCrackin, Raymond F. Schinazi, Peter B. Hill, Thomas W. Vahlenkamp, Mary B. Tompkins, Katrin Hartmann
Abstract: OBJECTIVE: To compare cytotoxic effects and antiviral efficacy of 9 nucleoside reverse transcriptase inhibitors (NRTIs) against FIV in feline peripheral blood mononuclear cells. SAMPLE: Peripheral blood mononuclear cells obtained from 3 specific pathogen-free cats. PROCEDURES: 3 of the 9 NRTIs had not been previously assessed in feline cell lines. Cytotoxic effects were determined by colorimetric quantification of a formazan product resulting from bioreduction of a tetrazolium reagent by viable peripheral blood mononuclear cells; uninfected cells from 1 cat were used in these assays. Cells from all 3 cats were infected with a pathogenic clone of FIV, and in vitro antiviral efficacy of each NRTI was assessed with an FIV p24 antigen capture ELISA. RESULTS: Cytotoxic effects in feline peripheral blood mononuclear cells were observed only at concentrations > 10 μM for all 9 NRTIs. Comparison of the cytotoxic effect at the highest concentration investigated (500 μM) revealed that didanosine and amdoxovir were significantly less toxic than abacavir. All drugs induced a dose-dependent reduction of FIV replication. At the highest concentration investigated (10 μM), there was no significant difference in antiviral efficacy among the test compounds. CONCLUSIONS AND CLINICAL RELEVANCE: The evaluated NRTIs had low cytotoxicity against feline peripheral blood mononuclear cells and appeared to be safe options for further in vivo evaluation for the treatment of FIV-infected cats. There was no evidence suggesting that the newly evaluated compounds would be superior to the existing NRTIs for reducing FIV burden of infected cats.
Keywords: Animals
Leukocytes, Mononuclear
Cell Line
Immunodeficiency Virus, Feline
Antiviral Agents
Reverse Transcriptase Inhibitors
Rights: © 2014 American Veterinary Medical Association
DOI: 10.2460/ajvr.75.3.273
Appears in Collections:Animal and Veterinary Sciences publications
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