Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Nedd4-2 (NEDD4L) controls intracellular Na⁺ -mediated activity of voltage-gated sodium channels in primary cortical neurons|
|Other Titles:||Nedd4-2 (NEDD4L) controls intracellular Na(+) -mediated activity of voltage-gated sodium channels in primary cortical neurons|
|Citation:||Biochemical Journal, 2014; 457(1):27-31|
|Jenny A. Ekberg, Natasha A. Boase, Grigori Rychkov, Jantina Manning, Philip Poronnik and Sharad Kumar|
|Abstract:||Nedd4-2, a HECT (homologous with E6-associated protein C-terminus)-type ubiquitin protein ligase, has been implicated in regulating several ion channels, including Navs (voltage-gated sodium channels). In Xenopus oocytes Nedd4-2 strongly inhibits the activity of multiple Navs. However, the conditions under which Nedd4-2 mediates native Nav regulation remain uncharacterized. Using Nedd4-2-deficient mice, we demonstrate in the present study that in foetal cortical neurons Nedd4-2 regulates Navs specifically in response to elevated intracellular Na+, but does not affect steady-state Nav activity. In dorsal root ganglia neurons from the same mice, however, Nedd4-2 does not control Nav activities. The results of the present study provide the first physiological evidence for an essential function of Nedd4-2 in regulating Navs in the central nervous system.|
|Keywords:||neuron; patch-clamp technique; sodium transport; ubiquitin protein ligase|
|Rights:||© The Authors Journal compilation © 2014 Biochemical Society|
|Appears in Collections:||Aurora harvest 7|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.