Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/8977
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Ex vivo cultre of peripheral blood CD34+ cells: effects of hematopoietic growth factors on production of neutrophilic precursors. |
Author: | Makino, S. Haylock, D. Dowse, T. Trimboli, S. Niutta, S. To, L. Juttner, C. Simmons, P. |
Citation: | Stem Cells and Development, 1997; 6(5):475-489 |
Publisher: | MARY ANN LIEBERT INC PUBL |
Issue Date: | 1997 |
ISSN: | 1061-6128 |
Statement of Responsibility: | Shigeyoshi Makino, David N. Haylock, Tracey Dowse, Sandra Trimboli, Silvana Niutta, Luen B. To, Christopher A. Juttner and Paul J. Simmons |
Abstract: | A major potential application for ex vivo culture of hematopoietic progenitor cells is the treatment of cytopenia following high-dose chemotherapy and hematopoietic transplantation. We have previously postulated that infusion of a sufficient number of neutrophil postprogenitor cells generated by ex vivo culture of CD34+ cells may be able to abrogate neutropenia. In this article, we describe further development of an efficient stromal-free, cytokine-dependent, static culture system for generation of these cells. Our previous studies indicated that maximal production of nucleated cells and myeloid progenitor cells from PB CD34+ cells occurred with multiple hematopoietic growth factor (HGF), notably the 6-HGF combination of interleukin (IL)-1, IL-3, IL-6, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), and stem cell factor (SCF). In the present study, we determine the contribution of each of these 6 HGF in generation of neutrophilic precursors. SCF, G-CSF, and IL-3 were found to be the most important HGF for production of neutrophilic cells. The 4-HGF combination of IL-3, IL-6, G-CSF, and SCF was optimized by performing dose-response experiments and shown to be as potent as 6 HGF for production of nascent CFU-GM and neutrophilic precursors. |
Keywords: | Neutrophils Hematopoietic Stem Cells Humans Antigens, CD34 Hematopoietic Cell Growth Factors Cytokines Hematopoietic Stem Cell Mobilization Cell Culture Techniques Cell Differentiation |
DOI: | 10.1089/scd.1.1997.6.475 |
Published version: | http://dx.doi.org/10.1089/scd.1.1997.6.475 |
Appears in Collections: | Aurora harvest Medicine publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.