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|Title:||Ex vivo cultre of peripheral blood CD34+ cells: effects of hematopoietic growth factors on production of neutrophilic precursors.|
|Citation:||Stem Cells and Development, 1997; 6(5):475-489|
|Publisher:||MARY ANN LIEBERT INC PUBL|
|Shigeyoshi Makino, David N. Haylock, Tracey Dowse, Sandra Trimboli, Silvana Niutta, Luen B. To, Christopher A. Juttner and Paul J. Simmons|
|Abstract:||A major potential application for ex vivo culture of hematopoietic progenitor cells is the treatment of cytopenia following high-dose chemotherapy and hematopoietic transplantation. We have previously postulated that infusion of a sufficient number of neutrophil postprogenitor cells generated by ex vivo culture of CD34+ cells may be able to abrogate neutropenia. In this article, we describe further development of an efficient stromal-free, cytokine-dependent, static culture system for generation of these cells. Our previous studies indicated that maximal production of nucleated cells and myeloid progenitor cells from PB CD34+ cells occurred with multiple hematopoietic growth factor (HGF), notably the 6-HGF combination of interleukin (IL)-1, IL-3, IL-6, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), and stem cell factor (SCF). In the present study, we determine the contribution of each of these 6 HGF in generation of neutrophilic precursors. SCF, G-CSF, and IL-3 were found to be the most important HGF for production of neutrophilic cells. The 4-HGF combination of IL-3, IL-6, G-CSF, and SCF was optimized by performing dose-response experiments and shown to be as potent as 6 HGF for production of nascent CFU-GM and neutrophilic precursors.|
Hematopoietic Stem Cells
Hematopoietic Cell Growth Factors
Hematopoietic Stem Cell Mobilization
Cell Culture Techniques
|Appears in Collections:||Aurora harvest|
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