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Type: Journal article
Title: The interaction of GM-CSF and IL-3 with the common beta chain of their receptors
Author: Bagley, C.
Woodcock, J.
Hercus, T.
Shannon, M.
Lopez, A.
Citation: Journal of Leukocyte Biology, 1995; 57(5):739-746
Publisher: Liss
Issue Date: 1995
ISSN: 0741-5400
Abstract: Human granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL-3) are cytokines active in both normal and abnormal hemopoiesis, inflammation, and immunity. Their biological activity is mediated via receptors that comprise a ligand-specific alpha chain and a beta chain that is common to the GM-CSF, IL-3, and IL-5 receptors. To understand the mechanism of action of GM-CSF and IL-3 in both normal and pathological conditions, we are seeking to define the structural elements required for ligand/receptor and receptor/receptor contact and their role in cellular activation. To this end we have identified a conserved motif in the first helix of GM-CSF, Glu21 that is critical for high affinity binding and biological activity. Charge-reversal mutagenesis of this residue generates a GM-CSF analogue that is devoid of biological activity and can antagonize the activity of wild-type GM-CSF. This probably results from the selective deficiency in interaction with the beta chain of the receptor and suggests that similar antagonists for IL-3 and IL-5 are also feasible. Complementary mutagenesis studies on the receptor beta chain have identified the putative B'-C' loop in the membrane-proximal domain as being critical for the high affinity binding of GM-CSF but not IL-3. Characterization of the specificity of sites of interaction between the ligands and receptors may permit the design of specific or genetic antagonists that may have important therapeutic implications.
Keywords: Humans
Growth Hormone
Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Interleukin-3
Sequence Alignment
Binding Sites
Binding, Competitive
Amino Acid Sequence
Protein Structure, Secondary
Sequence Homology, Amino Acid
Structure-Activity Relationship
Models, Molecular
Molecular Sequence Data
DOI: 10.1002/jlb.57.5.739
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