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Type: Journal article
Title: A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed
Author: Fuchs, J.
Neuberger, T.
Rolletschek, H.
Schiebold, S.
Nguyen, T.
Borisjuk, N.
Börner, A.
Melkus, G.
Jakob, P.
Borisjuk, L.
Citation: Plant Physiology, 2013; 161(2):583-593
Publisher: American Society of Plant Biologists
Issue Date: 2013
ISSN: 0032-0889
Statement of
Johannes Fuchs, Thomas Neuberger, Hardy Rolletschek, Silke Schiebold, Thuy Ha Nguyen, Nikolai Borisjuk, Andreas Börner, Gerd Melkus, Peter Jakob, and Ljudmilla Borisjuk
Abstract: While often thought of as a smoking drug, tobacco (Nicotiana spp.) is now considered as a plant of choice for molecular farming and biofuel production. Here, we describe a noninvasive means of deriving both the distribution of lipid and the microtopology of the submillimeter tobacco seed, founded on nuclear magnetic resonance (NMR) technology. Our platform enables counting of seeds inside the intact tobacco capsule to measure seed sizes, to model the seed interior in three dimensions, to quantify the lipid content, and to visualize lipid gradients. Hundreds of seeds can be simultaneously imaged at an isotropic resolution of 25 µm, sufficient to assess each individual seed. The relative contributions of the embryo and the endosperm to both seed size and total lipid content could be assessed. The extension of the platform to a range of wild and cultivated Nicotiana species demonstrated certain evolutionary trends in both seed topology and pattern of lipid storage. The NMR analysis of transgenic tobacco plants with seed-specific ectopic expression of the plastidial phosphoenolpyruvate/phosphate translocator, displayed a trade off between seed size and oil concentration. The NMR-based assay of seed lipid content and topology has a number of potential applications, in particular providing a means to test and optimize transgenic strategies aimed at the manipulation of seed size, seed number, and lipid content in tobacco and other species with submillimeter seeds.
Keywords: Plants, Genetically Modified; Brassica; Tobacco; Seeds; Lipids; Oils; Plant Proteins; Imaging, Three-Dimensional; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Reproducibility of Results; Lipid Metabolism
Rights: © 2013 American Society of Plant Biologists. All Rights Reserved.
RMID: 0030020023
DOI: 10.1104/pp.112.210062
Appears in Collections:Agriculture, Food and Wine publications

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