Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Inhibition of the Pim1 oncogene results in diminished visual function
Author: Yin, J.
Shine, L.
Raycroft, F.
Deeti, S.
Reynolds, A.
Ackerman, K.
Glaviano, A.
O'Farrell, S.
O'Leary, O.
Kilty, C.
Kennedy, C.
McLoughlin, S.
Rice, M.
Russell, E.
Higgins, D.
Hyde, D.
Kennedy, B.
Citation: PLoS One, 2012; 7(12):e52177-1-e52177-25
Publisher: Public Library of Science
Issue Date: 2012
ISSN: 1932-6203
Statement of
Jun Yin, Lisa Shine, Francis Raycroft, Sudhakar Deeti, Alison Reynolds, Kristin M. Ackerman, Antonino Glaviano, Sean O'Farrell, Olivia O'Leary, Claire Kilty, Ciaran Kennedy, Sarah McLoughlin, Megan Rice, Eileen Russell, Desmond G. Higgins, David R. Hyde, Breandan N. Kennedy
Abstract: Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3-5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors result in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.
Keywords: Animals
Rights: © 2012 Yin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0052177
Appears in Collections:Aurora harvest 2
Genetics publications

Files in This Item:
File Description SizeFormat 
hdl_90004.pdfPublished version11.52 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.