Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/90036
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Type: Journal article
Title: Transcriptome analysis of thermogenic Arum concinnatum reveals the molecular components of floral scent production.
Author: Onda, Y.
Mochida, K.
Yoshida, T.
Sakurai, T.
Seymour, R.
Umekawa, Y.
Pirintsos, S.
Shinozaki, K.
Ito, K.
Citation: Scientific Reports, 2015; 5(1):8753-1-8753-12
Publisher: Nature Publishing Group
Issue Date: 2015
ISSN: 2045-2322
2045-2322
Statement of
Responsibility: 
Yoshihiko Onda, Keiichi Mochida, Takuhiro Yoshida, Tetsuya Sakurai, Roger S. Seymour, Yui Umekawa, Stergios Arg Pirintsos, Kazuo Shinozaki, Kikukatsu Ito
Abstract: Several plant species can generate enough heat to increase their internal floral temperature above ambient temperature. Among thermogenic plants, Arum concinnatum shows the highest respiration activity during thermogenesis. However, an overall understanding of the genes related to plant thermogenesis has not yet been achieved. In this study, we performed de novo transcriptome analysis of flower organs in A. concinnatum. The de novo transcriptome assembly represented, in total, 158,490 non-redundant transcripts, and 53,315 of those showed significant homology with known genes. To explore genes associated with thermogenesis, we filtered 1266 transcripts that showed a significant correlation between expression pattern and the temperature trend of each sample. We confirmed five putative alternative oxidase transcripts were included in filtered transcripts as expected. An enrichment analysis of the Gene Ontology terms for the filtered transcripts suggested over-representation of genes involved in 1-deoxy-d-xylulose-5-phosphate synthase (DXS) activity. The expression profiles of DXS transcripts in the methyl-d-erythritol 4-phosphate (MEP) pathway were significantly correlated with thermogenic levels. Our results suggest that the MEP pathway is the main biosynthesis route for producing scent monoterpenes. To our knowledge, this is the first report describing the candidate pathway and the key enzyme for floral scent production in thermogenic plants.
Keywords: Arum; Flowers; Terpenes; Oxidoreductases; Transferases; Mitochondrial Proteins; Plant Proteins; Gene Expression Profiling; Temperature; Gene Expression Regulation, Plant; Energy Metabolism; Biosynthetic Pathways; Gene Ontology; Odorants
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
RMID: 0030024230
DOI: 10.1038/srep08753
Grant ID: http://purl.org/au-research/grants/arc/DP0771854
Appears in Collections:Earth and Environmental Sciences publications

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