Please use this identifier to cite or link to this item:
|Title:||Effects of Tumor Necrosis Factor-a, Interleukin 1B, and Activated Peripheral Blood Mononuclear Cells on the Expression of Adhesion Molecules and Recruitment of Leukocytes in Rheumatoid Synovial Xenografts in SCID Mice|
|Citation:||Journal of Rheumatology, 1999; 26(9, September 1999):1877-1889|
|Publisher:||J RHEUMATOL PUBL CO|
|Abstract:||<h4>Objective</h4>To examine expression of adhesion molecules within human synovial xenografts in vivo after injection of human cytokines or peripheral blood mononuclear cells (PBMC).<h4>Methods</h4>Rheumatoid synovium was transplanted into subcutaneous pouches in the ears of severe combined immunodeficient (SCID) mice. Tumor necrosis factor-alpha (TNF-alpha), interleukin 1beta (IL-1beta), or PBMC were injected into the xenografts. The grafts were assessed by immunohistochemistry and quantitative video image analysis.<h4>Results</h4>TNF-alpha, IL-1beta, and concanavalin A activated PBMC increased the expression of vascular cellular adhesion molecule 1 and intercellular adhesion molecule 1 significantly on vessels and nonvascular synovial cells compared with injection of medium only. Vascular expression of E-selectin, but not of MHC Class II, was also increased. These stimuli also induced the migration of murine leukocytes into the xenografts.<h4>Conclusion</h4>Rheumatoid synovial xenografts in SCID mice respond by upregulation of adhesion molecule expression when challenged in vivo with proinflammatory cytokines or activated PBMC.|
Disease Models, Animal
Tumor Necrosis Factor-alpha
Intercellular Adhesion Molecule-1
Vascular Cell Adhesion Molecule-1
Sensitivity and Specificity
|Appears in Collections:||Aurora harvest|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.