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Type: Journal article
Title: Quantitative proteomics reveals that plasma membrane microdomains from poplar cell suspension cultures are enriched in markers of signal transduction, molecular transport, and callose biosynthesis
Author: Srivastava, V.
Malm, E.
Sundqvis, G.
Bulone, V.
Citation: Molecular and Cellular Proteomics, 2013; 12(12):3874-3885
Publisher: American Society for Biochemistry and Molecular Biology
Issue Date: 2013
ISSN: 1535-9476
Statement of
Vaibhav Srivastava, Erik Malm, Gustav Sundqvist, and Vincent Bulone
Abstract: The plasma membrane (PM) is a highly dynamic interface that contains detergent-resistant microdomains (DRMs). The aim of this work was to determine the main functions of such microdomains in poplar through a proteomic analysis using gel-based and solution (iTRAQ) approaches. A total of 80 proteins from a limited number of functional classes were found to be significantly enriched in DRM relative to PM. The enriched proteins are markers of signal transduction, molecular transport at the PM, or cell wall biosynthesis. Their intrinsic properties are presented and discussed together with the biological significance of their enrichment in DRM. Of particular importance is the significant and specific enrichment of several callose [(1 → 3)-β-glucan] synthase isoforms, whose catalytic activity represents a final response to stress, leading to the deposition of callose plugs at the surface of the PM. An integrated functional model that connects all DRM-enriched proteins identified is proposed. This report is the only quantitative analysis available to date of the protein composition of membrane microdomains from a tree species.
Keywords: Membrane Microdomains; Cell Wall; Populus; Glucans; Isoenzymes; Glucosyltransferases; Cell Culture Techniques; Proteomics; Signal Transduction; Gene Expression Regulation, Plant; Biological Transport; Mass Spectrometry; Stress, Physiological; Molecular Sequence Annotation; Plant Cells
Rights: © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
RMID: 0030021019
DOI: 10.1074/mcp.M113.029033
Appears in Collections:Agriculture, Food and Wine publications

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