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Type: Book chapter
Title: Temperature Switch PCR (TSP): a gel-based molecular marker technique for investigating single nucleotide polymorphisms
Author: Thanh, L.
Khoo, K.
Citation: Crop breeding: methods and protocols, 2014 / Fleury, D., Whitford, R. (ed./s), Ch.3, pp.37-46
Publisher: Humana Press
Issue Date: 2014
Series/Report no.: Methods in Molecular Biology; 1145
ISBN: 1493904450
Statement of
Le Phuoc Thanh and Kelvin Khoo
Abstract: Temperature Switch PCR (TSP) is a robust single-marker single nucleotide polymorphism (SNP) genotyping technique with broad applications in genetic studies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.
Keywords: TSP; SNP; Locus-specific primers; Gel electrophoresis; Molecular marker
Rights: © Springer Science+Business Media New York 2014
RMID: 0030027997
DOI: 10.1007/978-1-4939-0446-4_3
Appears in Collections:Agriculture, Food and Wine publications

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