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Type: Book chapter
Title: Caspase-2 Protocols
Author: Dorstyn, L.
Kumar, S.
Citation: Methods in Molecular Biology, 2014, pp.71-87
Publisher: Springer New York
Issue Date: 2014
Series/Report no.: Methods in Molecular Biology; 1133
ISBN: 9781493903566
Statement of
Loretta Dorstyn and Sharad Kumar
Abstract: Caspase-2 has been shown to function in apoptosis and in some non-apoptotic pathways, including tumor suppression and aging. Caspase-2 has some unique features and is the only caspase that constitutively localizes to the nucleus, although its nuclear function remains unknown. During apoptosis signaling, caspase-2 rapidly homodimerizes, which leads to its activation and proteolytic processing. The activation of caspase-2 can be measured by assessing its dimerization and/or cleavage of the caspase-2 zymogen and its substrates. This chapter outlines commonly used methods to purify recombinant caspase-2 and assess its activity and function in vitro and in cultured cells or tissue extracts.
Keywords: Apoptosis; Caspase-2; Caspase activity; Peptide substrates; Recombinant protein; Immunoblotting
Rights: © Springer Science+Business Media New York 2014
DOI: 10.1007/978-1-4939-0357-3_4
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