Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Citation:||Caspases, paracaspases and metacaspases: methods and protocols, 2014 / Bozhkov, P., Salvasen, G. (ed./s), vol.1133, Ch.4, pp.71-87|
|Series/Report no.:||Methods in Molecular Biology; 1133|
|Loretta Dorstyn and Sharad Kumar|
|Abstract:||Caspase-2 has been shown to function in apoptosis and in some non-apoptotic pathways, including tumor suppression and aging. Caspase-2 has some unique features and is the only caspase that constitutively localizes to the nucleus, although its nuclear function remains unknown. During apoptosis signaling, caspase-2 rapidly homodimerizes, which leads to its activation and proteolytic processing. The activation of caspase-2 can be measured by assessing its dimerization and/or cleavage of the caspase-2 zymogen and its substrates. This chapter outlines commonly used methods to purify recombinant caspase-2 and assess its activity and function in vitro and in cultured cells or tissue extracts.|
|Keywords:||Apoptosis; Caspase-2; Caspase activity; Peptide substrates; Recombinant protein; Immunoblotting|
|Rights:||© Springer Science+Business Media New York 2014|
|Appears in Collections:||Aurora harvest 2|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.