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Type: Book chapter
Title: A combined free flow electrophoresis and DIGE approach to compare proteins in complex biological samples
Author: Fung, K.Y.C.
Cursaro, C.
Lewanowitsch, T.
Cosgrove, L.
Hoffmann, P.
Citation: Protein electrophoresis: methods and protocols, 2012 / Kurien, B.J., Scofield, R.H. (ed./s), vol.869, Ch.12, pp.135-146
Publisher: Humana Press
Issue Date: 2012
Series/Report no.: Methods in Molecular Biology; 869
ISBN: 9781617798214
Editor: Kurien, B.J.
Scofield, R.H.
Statement of
Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann
Abstract: Free flow electrophoresis (FFE) has been applied in numerous studies as a protein separation technique due to its multiple advantages such as fast and efficient sample recovery, high resolving power, high reproducibility, and wide applicability to protein classes. As a stand-alone platform however, its utility in comparative proteomic analysis is limited as protein samples must be run sequentially rather than simultaneously which introduces inherent variability when attempting to perform quantitative analysis. Here we describe an approach combining fluorescent CyDye technology (DIGE) with FFE to simultaneously separate and identify differentially expressed proteins in a model cell system.
Keywords: Free flow electrophoresis
Rights: © Springer Science+Business Media B.V. 2012
DOI: 10.1007/978-1-61779-821-4_12
Appears in Collections:Aurora harvest 2
Molecular and Biomedical Science publications

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